The product could potentially be toxic, resulting in a lethal effect on the cells. However, alterations in the incubation temperature or the pH of the culture could potentially mitigate these effects.

Thanks for your answer, The incubation temperature of the expression period is 16 C.

Well, you might conduct experiments at temperatures of 20°C, 25°C, 30°C, and 37°C for example. For each of these temperatures, you could prepare cultures at three different pH levels: 7, 8, and 9. This approach would allow you to observe the effects of these variables on your culture and protein expression