I have animal feed sample. I am using 1 g feed and 5 ml buffer. After protein extraction. I diluted my sample 20 and 40 times. but the concentration and Percentage of protein varies in both dilutions but sample is same. For example, for 20 times dilution, i got 12% and for 40 times dilution, i got 14 %. I need same values for every dilution of same sample.
12% versus 14% may not be a statistically significant difference, depending on the experimental variation. If you made replicate measurements at each dilution, what were the means and standard deviations of the measurements?
If the difference is statistically significant, then you should consider possible technical reasons for it, such as in the way the samples were taken for dilution. A 200 mg/mL suspension may be difficult to accurately dispense. Was there any residue left in the pipette? Was the sample uniformly mixed?
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