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I need help using a microplate reader to measure the intensity of a fluorescent dye. Could you provide any recommendations and instructions for doing this?
25 November 2024 6,219 1 View
We are a Chinese education company dedicated to helping our clients publish SCI, SCIE, SSCI WoS indexes quickly. We have a wide range of paper orders in various fields and we are actively looking...
19 November 2024 1,708 1 View
If DNA is complexed with cationic liposomes, does it stay complexed on the surface or there's some kind of rearrangement of the structure? Thanks
12 November 2024 4,621 2 View
I'm currently optimizing my primers at various annealing temperatures. Initially, I achieved bands at the target product size using a specific DNA sample. However, when I applied the same...
02 November 2024 9,828 4 View
Blast furnace Taphole protection 1500 m3
01 November 2024 8,698 2 View
NK cells release a large amount of IL-10 and TGF - β in the microenvironment of malignant tumors, leading to immune environment imbalance and inhibiting the effective immune response of NK cells....
30 October 2024 2,478 0 View
What is the effect of the depth of a microfluidic chip based on hydrodynamic focusing on liposome particle size?
22 October 2024 6,451 0 View
I've used the cut and extrude options in the Parts module and the cut operation in the Assembly module. However, when I assemble the soil and pile, there are small gaps or overlaps at the...
15 October 2024 5,355 2 View
Which is easier to use for observing DNA oligonucleotides on AFM: a fluid cell or a mica substrate?
01 October 2024 8,275 0 View
I am a matron for an operating theatre and I am having a hard time finding the right topic for my research proposal. I wonder if you could help me find a simple one, please?
29 September 2024 7,913 1 View
What criteria a supporting electrolyte should satisfy for using it for detection of metal ions using CV
27 September 2024 6,895 2 View
Blast Furnace
23 September 2024 3,317 2 View
I am basically working with self-assembled VLPs. I am not sure if His tag in my protein is not exposed (I can detect in reducing western blot by his tag antibody) or other technical issues, and...
16 September 2024 7,690 3 View
Are there some examples of Carbonatites found outside Continental areas, i.e, Island Arcs and/or Oceanic Crusts origin ?? In this case, can these resources hold Li, REE, LREE and related ?...
03 September 2024 4,863 1 View
Hello everyone, In my lab, we isolate mononuclear cells (MNC) from umbilical cord blood (UCB) using density-gradient isolation (Ficoll-Paque), use NH4Cl to remove erythrocytes, and then we...
02 September 2024 3,454 0 View
I would like to synthesize the t-BuSH by using NaSH but cannot get the product. I would be happy to get any suggestions or procedures to get t-BuSH. Thank you, Alok
31 August 2024 8,713 0 View
We have the BXPC-3 cell line in passage 2 but when we do a few passages the cells change their cell morphology from epithelial to fibroid type. I don't know if anyone has had a similar problem and...
30 August 2024 2,633 2 View
My western blot is not coming properly. I am assuming that due to overnight transfer the protein band is not coming in chemidoc Biorad. Or when coming too much hazy it is. Can anyone please help...
30 August 2024 9,573 1 View
what would be the easiest method to make the glass coverslip hydrophobic
27 August 2024 8,925 2 View
Hello to all friends I want to remove the lithium oxide from the lithium coin in the glove box. Please suggest me a method other than using sandpaper or a cutter blade? Please suggest an article...
25 August 2024 4,345 5 View
Hi all, I am very new to cell cultures and cytotoxicity tests. I am currently planning my MTT experiment using cells that will be treated with rutin and gallic acid. In the 96-wells, how does one...
22 August 2024 4,386 1 View
I have made a polyoxometallate material for a cathode in an aqueous sodium battery. I have deposited it on FTO glass, and I will use Pt wire as the counter electrode and Ag/AgCl as a reference...
20 August 2024 9,193 7 View
Hi! I am growing intestinal epithelial cells in 24-well transwell plates with 8um pores. I am using Caco-2 or T84 cells. I find it difficult to visually assess the monolayer using light...
20 August 2024 8,870 5 View
Dear all, I have tried to use a mammalian Expression Vector System to overexpress a protein of interest in AGS cell line. Cells were selected based on puromycin resistance conferred by successful...
19 August 2024 5,914 2 View