Contact experts in Drugs to get answers
1,490 views 2,938 posts
Questions related to Drugs
I have got a drug that contain :pregelatinized tapoioca starch, lactose, monohydrate, stearic acid, magnesium stearate, iron oxide (E172).how can purify it in order to use it in the syntheis of...
14 April 2022 5,347 3 View
Hi, I want to test our anti-tumor drug in the B16F10 tumor model, but the fast-growing nature of this tumor makes the observation period very short, so I wonder if there is a chemo that can delay...
14 April 2022 9,642 1 View
Hi everyone, I am working on developing alginate/pectin beads in CaCl2 using ionic gelation method. The problem I am having is the low loading efficiency. I have tried all possible trials like,...
13 April 2022 2,960 7 View
What could be a possible reason if a drug not bind with BSA? I have used different BSA concentration and different complex concentration with different incubation time but all in vain.
09 April 2022 8,674 1 View
Hi, I want to ask about Indirect ELISA. As you in pictures, some wells did not turn blue after adding solution B and also same wells did not turn yellow after adding stop solution. İs that normal...
07 April 2022 7,476 3 View
While formulating liposomes, I use soybean lecithin as a phospholipid. what is the ideal phosphatidylcholine content in highly pure soybean lecithin that assure it’s solubility in ethanol,...
05 April 2022 9,798 3 View
I did the math using 66430.3 g/mol and it gives me 9.96g of BSA in 1 mL of water. This is not going to dissolve. I have looked at other papers and they use this concentration but they do not...
04 April 2022 3,230 2 View
Hi researchers! I want to run a mtt assay on PBMC to show that a cytotoxic drug in a leukemia, does not have any effect on normal cells I mean PBMC! Is there any special protocol to isolate ,seed...
03 April 2022 3,615 3 View
Hi, Im trying to do an MTT assay with UT7 cells ( in suspension). I start my asaay treating the cells with different EPO concentraions and another inhibitor. There are 4 96 well plates- 0-72...
03 April 2022 9,275 2 View
Hello everyone, I have recently started working with NGS data (amplicon sequencing) for phylogenetics and species-delimitation studies of closely-related specimens (typically within a genus or a...
03 April 2022 3,132 0 View
I performed an MTT assay to see the effect of an inhibitor on AGS cells. I seeded them on Day 1, added the inhibitor on Day 2 and performed the MTT assay on Day 3 (left the inhibitor act for 24...
01 April 2022 4,312 3 View
I'm treating cancer cells with two different cytotoxic combination drug with low concentrations (mtt assay). I have 5 concentration (serial dilution 1:2) of each drug in triplicates. Drug A, Drug...
30 March 2022 4,468 2 View
I am treating populations A and B with drug X and with drug Y. I get a significant difference in response to drug X between populations A and B via the Mann Whitney U test. Meanwhile, there is no...
29 March 2022 2,308 2 View
There is enough evidence to suggest increasing prevalence of misuse of prescription medications including not just Opiates and benzopdiazepines but also more recently Gabapeninoids. I am joining...
29 March 2022 1,962 3 View
Following differentiation experiment (24h PMA 100 ng/mL -> 48h RPMI 10% FBS -> 72h IL-4 40ng/mL (M2) or LPS 50 ng/mL + INF 50 ng/mL (M1)) we found very difficult to detach cells for FACS...
29 March 2022 1,147 1 View
I need to choose an endotoxin detection kit for my company. The objective is to use it to test for the presence of endotoxins in parenteral products and ppi water. The proposed kits are Charle...
27 March 2022 8,867 2 View
Can anyone point towards a tutorial or video where I can perform co-localization analysis using ImageJ? I have a very simple experiment where I have stained HEPG2 cells with DAPI in order to count...
27 March 2022 1,994 4 View
From finding the target gene for knock out to analyze the result, how much time will be needed? Test subject is rapeseed(Brassica napus). If anyone have the full manual kindly provide that.
24 March 2022 6,661 4 View
I have read some discussions here and other forums seems that some diabetic mice will experiencing decreased insulin expression. Some people suggested using proinsulin as the marker for insulin/...
20 March 2022 9,636 1 View
The Zn2 site of class B1 β-lactamase contains a conserved cysteine residue, Cys208 in NDM-1, required for Zn2+ binding. The disruption of Cys208 for Zn-coordination may effectively inhibit the...
19 March 2022 3,767 2 View
I have done a Logistic Regression model and I know that only the p-value and RR^2 are not enough. Could pls anyone suggest or any help?
18 March 2022 7,913 7 View
I was supposed to extrude ~100nm DPPC liposome via the Avanti extrusion kit after hydrating the liposome film. However, it gave me bigger LUV during several trials (around 130-150nm). Although it...
17 March 2022 5,616 2 View
Does a transmembrane receptor like leptin receptor do not have a signal peptide?
17 March 2022 1,927 2 View
I will perform MTT assays on cancer cells with 4 hours long treatments. Because of that, I would be finishing the MTTs too late, and my lab gets closed early. Can I leave the formazan crystals...
16 March 2022 148 4 View