Trouble detecting organic acids with LC-RI detector?

27 April 2024 1 3K Report

I am trying to detect organic acids on my waters LC, which is equipped with a W410 refractor index detector. I am using a Aminex HPX-87H Column which is supposed to work to separate organic acids. I’m not sure if I’m missing something in my method settings but I am not detecting anything at all. If anyone has any insight that would be greatly appreciated

Paul Körner

Both, HPX-87H column and RI detector should be suitable for separation and detection of organic acids. However, I don't know either, if something is missing in method as I don't know your method. If you could provide some more information like solvent composition, standard concentrations, maybe a chromatogram, that'd be helpful.

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