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24 May 2024 5,071 2 View
How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...
24 May 2024 7,156 2 View
Can data that includes less than values (below the limit of detection in chemical analysis) be used in ANOVA if the data is re-coded in some way? Perhaps using the actual detection limit or half...
24 May 2024 7,240 3 View
The desired gene region was amplified by PCR and the resulting PCR products were cleaned. Bands were observed in an agarose gel and measured in a spectrophotometer. Then, transcription was...
23 May 2024 6,871 1 View
Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...
23 May 2024 7,427 0 View
I'm trying to synthesize a highly polar compound which is uv inactive (In TLC charring at the bottom- 0.1RF/20%MeOH:DCM ,MP) . I have confirmed the compound by simple mass and H'NMR spectrums but...
23 May 2024 9,878 3 View
I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...
22 May 2024 8,694 4 View
In this online file you have included my last 2 publications. But your original file my (Mohammad Ansaruzzaman, student of free university Brussels & Karolinska Institute) you have included...
22 May 2024 147 1 View
Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...
22 May 2024 1,401 0 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
22 May 2024 4,427 0 View
I am looking for the optimized protocol for protoplast isolation by using just expanded unifoliate leaf of Mung Bean.
22 May 2024 3,634 1 View
I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities
21 May 2024 714 4 View
Suppose I use "pBABE-puro-GFP-wt-GeneX" to get GeneX expressing with GFP (I am not sure whether it will replace the endogenous GeneX or get inserted somewhere else since the pBABE-puro vector uses...
20 May 2024 4,792 0 View
How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution
18 May 2024 3,727 2 View
When using different laser energies, what is the effect on the size of nanoparticles? Does it increase or decrease with increasing energy? Please help
16 May 2024 4,756 2 View
In FTIR spectrum, Mo-S vibrational mode appears at lower wavenumber (around 468 cm-1)whereas W-S vibrational mode appears at higher wavenumber (580 cm-1) , which is against hooke's law. what is...
16 May 2024 1,279 0 View
I've been working on graphene ink for a while now and have encountered some issues with its spreading on paper. I'm uncertain whether the problem lies in the ink formulations or the size of the...
14 May 2024 9,250 2 View
Most times after checking the cyctotoxity, my results are not consistent. But i used the same concentration of my stock solution. Can anyone help out with how to determine the concentration of my...
10 May 2024 1,597 2 View
A population afflicted with liver disease, putting them at risk of cancer, undergoes surveillance to detect cancer at its earliest stages. The recommended protocol involves ultrasound screenings...
08 May 2024 9,412 0 View
What strategies can healthcare providers employ to maintain optimal glucose control in diabetic patients undergoing surgery?The Impact of Hyperglycemia on Perioperative Outcomes in Pat...
08 May 2024 7,120 1 View
Three of my samples have negative percent inhibition while the other three samples have positive percent inhibition. For the negative values, the absorbance of the samples are higher than that of...
07 May 2024 4,531 0 View
My study population can have either or both reactive and fasting hypoglycemia for different founder variant genetic causes. I want to use the onset of symptoms to separate and determine the...
03 May 2024 1,824 0 View
Is it safe to use plastic pippette or only glass pippette can use?
02 May 2024 799 5 View
When I specify the number of processors = 4, my calculation works fine. But when I want to specify the number of processors more than four, the program gives: "Will use up to 8 processors via...
01 May 2024 3,143 1 View