How do I verify age and language capabilities when making use of data collection via online questionnaires?

Our inclusion criteria are that participants must be 18 years or older and that they must be able to read and understand English/Afrikaans in order to take part in the study. However, the informed...

02 March 2018 6,995 3 View

Suggestions for reliable online questionnaires to evaluate neuropsychiatric disorder symptoms?

Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?

09 October 2017 425 4 View

Are there labs in Lyon, France, working on neuroscience & epigenetics?

I will be visiting Lyon in October and is interested to visit some labs there that preferably work in the behavioural neuroscience/neuro genetics/microbiome/transciptomics/epigenetics fields. Just...

08 September 2014 6,684 5 View

Which kits are best to use for isolating microbial DNA from stool samples?

06 July 2014 5,655 15 View

What is the best protocol for extraction of high quality RNA from Oragene samples?

We are having trouble extracting RNA from Oragene samples; we have very low 260:230 ratios and certain samples have very low concentrations. We have tried the RNAeasy kit, the trizol and a...

01 February 2014 6,115 2 View

Dilutions of precursor miRNA and 3'UTR target for co-transfection in luciferase experiment?

I want to make sure if my calculations are correct. I have 370ng/ul stock of my 3'UTR reporter plasmid, I have to use 10ng during the transfection, does that mean that I use 0.03ul of the stock...

07 August 2013 8,419 6 View

Should I differentiate my neuroscreen-1 cells before conducting functional miRNA analyses?

I have neuroscreen-1 (NS-1) cells (theses cell are sub-clones from PC12 cells) which I want to co-transfect with a specific miRNA as well as that miRNAs predicted mRNA target, to see if the miRNA...

06 July 2013 7,728 11 View

Products for functional miRNA experiments

Which products or brands would you recommend for performing miRNA luciferase assays to determine if an mRNA sequence is a direct target of the particular miRNA? Specifically relating to the miRNA...

05 June 2013 6,348 1 View

Program to predict CpG islands in gene bodies.

I would like to know what prediction programs are out there that predict CpG islands within gene bodies (not promoters).

01 February 2013 8,611 2 View

Freeze-drying miRNA samples to increase concentration

Has anyone freeze-dried their miRNA samples (in a separate small RNA fraction, not in total RNA) in order to increase the concentration? Does it affect the RNA integrity?

10 November 2012 334 14 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View