After drug and PI-stained flow cytometry, I found a peak between S and G2/M. Advise for alternative technique to elucidate the cell cycle stage?

These AML cell numbers are highest in G1 but move to G2/M with one of the drugs. Another of the drugs appears to induce a minor peak on the lower end of G2/M, and possibly into S-phase. When the...

22 July 2018 6,759 2 View

Help with nudibranch reproductive anatomy?

Hi, I am new to nudibranch dissection and I was wondering if anyone here could provide me with some guidance on identifying reproductive structures? I have attached a picture of the dissected...

02 June 2018 2,279 2 View

Could the equivalent control method of traditional SMC lead to a smaller dot(s) when controller working than other approaches of control law design?

Hello everyone, I am new to sliding mode control and interested in it very much. Today I come here with several questions, I hope you can give me a hand to my study, I am helpless and a little bit...

03 July 2017 2,974 11 View

What factors influence African and Caribbean women towards senior positions?

I am interested in the experiences of these women's journey towards senior positions in education.

28 March 2016 2,942 3 View

What are peoples opinion with regards to potential DNase contamination in a manufacturing facility? Have people every tested for DNase contamination?

13 May 2015 699 1 View

Does a defendant that has fetal alcohol syndrome have some protection in a misdimeanor court case?

The defendant was 16 years old and was doing graffiti and was also charged with possession of tobacco. The defendant had been diagnosed with fetal alcohol syndrome.

12 March 2015 3,807 10 View

Is anyone conducting research dealing with coaching models or reality TV?

I am interested in two types of research topics: One factors involved with successful coaching models used in couples or marriage coaching. Most coaches use Co-Active coaching, GROW or REGROW....

09 January 2015 8,887 3 View

Has anyone any information on ontogenetic colour changes in Sicydium (Gobiidae)?

A colleague and I just started working on Sicydium punctatum, and noticed several colour variations in what we think is this species.

03 February 2014 2,721 2 View

How can I d meta anaalysis on Binary data?

I have completed a systematic review and have collate 22 useful papers. i can see from previous meta analysis from this data set that authors have performed mata analysis onthe binary data (it is...

01 January 1970 1,915 3 View

Do Protests Diffuse to other Countries, and When?

In the November issue of the Journal of Conflict Resolution (https://tinyurl.com/tyhrn2j) Adrian Lucardi and I, debate with Bunce, Wolchik, Hale, Houle, Kayser, and Weyland, about whether...

01 January 1970 5,481 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View