How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

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17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

What are the causes of hyphae granulation?

Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...

05 February 2021 5,523 4 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

Is there any alternative of PEEK polymer for permanent implant application?

I am looking for an alternative of PEEK polymer having same characteristics like PEEK which can be used in permanent implant devices. Any help or suggestions are highly appreciated. Thanks.

03 January 2021 6,062 4 View

How do I use clinical BioBERT for relation extraction from clinical text?

Hola I'm working on a project that deals with clinical named entity recognition, relation extraction etc. I'm currently using Scispacy library for NER work. However, I'm searching for a open...

16 December 2020 5,701 1 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Does someone know which structure could exit the olfactory bulbs at their antero-dorsal top ?

Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...

27 January 2021 797 6 View

What cryostat temperature is optimal to collect frozen 14 micron thick vagus nerve sections?

Hi, I'm going to section vagus nerve tissue embedded in OCT, collecting 14 micron thick sections. What cryostat temperature is optimal for colleting these sections?

01 December 2020 4,400 2 View

293-hTRPV1 stable cell line ?

Hi, I have some trouble with my HEK293-hTRPV1 stable cell line. I made a 293-hTRPV1 stable cell line with Flp system from Thermofischer...

16 September 2020 5,243 2 View

How Hansen solubility parameters helps in drying fibers during electrospinning?

Hansen solubility parameters determine hoe a polymer is soluble in a the ifferent solvent. But how it helps in case of solidifying a fiber during electrospinning process?

14 September 2020 4,570 2 View

Hi everyone, I need to model and analyze a fiber reinforced rigid pavement, is it possible to suggest some ideas how to model FRC pavement?

I am looking for related modeling methods, software as we as helpful papers or books.

30 August 2020 6,104 1 View

Uncertainty on which statistical test to use and how to present my data best ?

Hi im currently writing my final year project up and am struggling on deciding which method of statitical analysis is best. I performed research on cricets and recorded the peaks per second of...

14 March 2020 213 3 View

How to measure nerve compound action potential ex-vivo in a "reproducible" way?

Hi all, I'm trying to measure the compound action potential (CAP) on an isolated porcine phrenic nerve which is stimulated on the proximal end but every time I remove the nerve from the electrode...

18 February 2020 4,831 2 View

Where i can find retinal nerve fiber layer defects fundus image database with ground truth table please?

i need fundus image dataset to continue my research, and its need to be classified, if any body can help me please.

01 February 2020 3,946 3 View

What could be the reason for a fiber shape TiO2 nanowire besed dye sensitized solar not to work?

I am trying to fabricate a fiber shape TiO2 nanowire-based dye-sensitized solar cell on Ti wire. I use a hydrothermal route to grow the TiO2 nanowires. Then I absorb the nanowires with N719 dye. I...

15 December 2019 1,407 3 View