I am currently planning an experiment making an antibody-siRNA conjugate using this kit: TriLink Antibody-Oligonucleotide All-in-One Conjugation Kit, https://www.trilinkbiotech.com/cart/scripts/prodView.asp?idproduct=14207
As each shot of this experiment is quite expensive, I'm looking for people who have already used this kit or similar approaches to ask for their experiences. The company claims this is working perfectly no matter if you use single or double stranded oligos, short or long sequences, DNA or RNA, 3' or 5'-end for conjugation… but as we know reality can often be more complicated.
So what I would be interested in is what type of oligo you used it for, how the success rate and yield were, if you used different kits or strategies and how they compare, which ratio of oligo to antibody you used and any practical tips you could offer. I'm especially interested in short double-stranded RNA, but experience with any kind of oligo would be helpful. Thanks a lot!
I haven't used this particular kit, but I have used TriLink many times and have never been disappointed with the results; they churn out some high quality products.
You don't necessarily need a kit to do this type of kit, you could just order the oligo from TriLink and request a Benzenesulfonyl Fluoride modification on either the 3' or 5' end, possibly with a small PEG spacer between (if you're using in-vivo then the other end will need to be capped to prevent exonuclease degradation). This type of oligo could then readily bind to any protein using a simple SuFEx click-chemistry protocol (heterolytic conjugation using soft reagents and irreversible unless subjected to extreme conditions).
I don't think they offer this in their catalog but I ask them for weird stuff all the time and they never have an issue making it for me.
Thanks, Vincent Paul Schmitz. I hadn't heard about this strategy before and after looking a bit into it, it seems to be a really interesting alternative. Also good to know that you had positive experiences with TriLink.
this video shows off a kit that will help you
https://www.youtube.com/watch?v=SJEulFA2m5c&list=PLTCBtzqkKa4QhazLdinhoIuIx-HV6YDCX
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- DNA