I have already amplified rbcl, matk, trnl-f, psba-trnh and ITS regions of my study plants. Now I will be doing sequencing of those regions. I have purified those PCR products by Quaquick gel extraction kit. Now what are the steps, I have to follow before loading onto sequencing?? We have BigDye terminator Kit
Can anybody kindly tell me the detailed steps of cycle sequencing and other procedure?