What are the possible problems with repeat sequence placement on the map and solutions to overcome?

Renuka Karunaneethy @Renuka_Karunaneethy2

06 June 2014 0 7K Report

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Science method

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Please give some informations on the Corynebacterium butyricum?

I would like to gather some information on the Corynebacterium butyricum such as its natural habitat, clinical significance for my study purpose thank you

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Apart from the potassium sodium tartrate, what other chemical can be use to prepare 3,5-Dinitrosalicylic acid reagent?

Is there any substituent for potassium sodium tartrate, in preparing the DNS reagent? thank you.

10 November 2014 9,507 2 View

How do you prepare 0.1M of citrate buffer at 7.0 pH?

I have to prepare 0.1M of citrate buffer to dilute glucosamine. What are the protocols for the preparation of 0.1M of citrate buffer at pH 7.0? Thank you.

10 November 2014 9,175 5 View

Can the Letendraea helminthicola exhibit chitinase activities?

I'm doing a research on on chitin detection . I'm just curious whether the Letendraea helminthicola can exhibit chitinase activities. Links of some journals will help for further readings. thank you

10 November 2014 9,719 2 View

What is the standardized method to prepare DNS reagent?

Please suggest me a standardized method to prepare DNS reagent for the determination of reducing sugar by using N acetyl D glucosamine. Thank you

10 November 2014 1,229 2 View

What are the methods to generate N-acetyl-beta-D-glucosamine curve?

I'm doing a experiment on chitinolytic assay of fungi isolates. Can anyone suggest from where I can get the methods to generate the standard curve of N acetyl beta D glucosamine. Thank you.

10 November 2014 5,402 5 View

What are the possible problems that may encounter during cloning a gene?

Currently, I'm doing research on problems that scientists may encounter during cloning gene. I found that mutations are one of the major problem. Any other suggestions? Thank you.

05 June 2014 9,506 8 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View