Does anyone have a protocol or experience with extracting genomic DNA from very small sample volumes of 10-30 microliters?
The sample is a microbial enrichment from a sewage treatment plant.
These are enrichments, but under very stringent conditions - I try to cultivate bacteria with doubling times of around 21 days. Therefore, I do not expect that there will be many organisms in my sample. The experiment is already finished and therefore I only have the samples once and when I start with the DNA isolation and nothing gets around, the samples are gone.
I want to do a metagenome analysis (shotgun illumina) with it but I am actually not sure what minimum amount is being needed - I guess 100 μg would be good but I am not sure about that.
Therefore I am currently looking for the best possible protocol before I start the isolation...
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