Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.
Hello Vinay, According to my experience, I have extracted DNA/RNA from different plant tissues and is very important to consider the plant species. Different vegetal tissues contain different cellular components (which can act as inhibitors of the PCR reaction). First all, check bibliography, you can consult papers where the authors made nucleic acid extraction from a vegetal tissue from the same species that are you working.
In any case, you can use CTAB extraction. This protocol is the "Gladiator" of all the DNA extraction protocols in plants. EPPO protocol for the detection of Xylella fastidiosa (Bacterium that have most that 500 differents plant hosts) recommends this protocol.
Links:
https://onlinelibrary.wiley.com/doi/epdf/10.1111/epp.12469
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC324241/
PD: If you continue to have problems with inhibitors, try diluting the DNA extracts. That way, the inhibitors are diluted and you can get a favorable PCR result.
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