Iwill be so grateful if any researecher can share me protocol of DNA extraxtion using SDS/PK/CTAB method .
thanks
Hello Ammam
I used this protocol for DNA extraction from rumen content which is mainly including feed and microorganisms. It worked very well for me.
Hello, you can find some info in this article
Article Optimization of high-yielding protocol for DNA extraction fr...
Hi Ammam,
If you want to prepare genomic DNA for PCR, which is known as 'dirty DNA preparation', you can use following protocol. It is cheap, fast and works really nice with PCR.
Collect your samples to a 1.5 ml Eppendorf tubes. In general add 200 ul of 50 mM
NaOH / 0.2 mM EDTA lysis solution and boiled at 99⁰C for 20 minutes. Chill the reaction on ice and quench it using 10 μl of 1M Tris-HCl, pH 7.5 (10 lysis solution: 1 quenching buffer). Use 0.6 -1.0 ul of the extract for PCR.
Protocol was obtained from 'G. E. Truett et al., Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). Biotechniques 29, 52, 54 (2000)'.
Good luck
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