Iam sorry but it looks like that your RNA is completly degraded and that you have only very small fragments left, as you see in most of the lanes a low molecular smear. Due to the complete degradation you don't have 18s or 16s bands. Even if your RNA is degraded it will still look good, if you measure this with the Nandrop as you have RNA in your sample and the Nanadrop will not distinguish between intact RNA and degraded RNA.

I don't think that you have any problems with the Gel electrophoresis itself as the bands of your ladder look good.

I am not sure what you want to do with the RNA but I think it is very likely that your downstram applications will not work very well - if at all - with these samples. My advice would be to isolate new RNA.

I think you should isolate new RNA because there isn't any problems with gel electrophoresis

Your isolated RNA was degraded, so you got a smeared band. Try to isolate RNA in a sterile environment: Incubate tips, vials, and tubes in DEPC water or RNAse-free water for 12 hours before isolation.

Your RNA extraction gel shows possible RNA degradation (smearing), genomic DNA contamination (high-molecular-weight bands), or low RNA yield (faint bands). This could be due to RNase contamination, poor lysis/homogenization, or insufficient DNase treatment. Ensure proper sample handling, use RNase-free conditions, and include DNase digestion. Check RNA purity via NanoDrop (260/280 & 260/230) and Bioanalyzer to confirm integrity. Nu Fa