I am trying to load an isoflavonoid which is hydrophobic in nature onto graphene oxide by simply mixing a 1:1 aqueous dispersion of graphene oxide with the isoflavonoid mixed with DMSO (Compound has high solubility in DMSO) for a period of 24 hours (As per reported literature). I cannot confirm the success of loading since the compound precipitates out in the aqueous phase giving me erroneous results when I take the UV-Vis absorbance readings. I want to remove the precipitate out by a simple DMSO washing but I am afraid that the loaded drug may also wash out since the mechanism of loading is via pi-pi stacking and hydrophobic interactions. I want to know how strong is pi-pi stacking and whether I can wash the precipitate out by DMSO washing.
Also if any changes in the protocol might help with the loading.
You coated graphene oxide in DMSO with a flavonoid. Then placed in water. The hydrophobic interaction in water is greater, therefore, the particles aggregate and precipitate. You need to stabilize the suspension by adding a small amount of Na OН, КОН. In an alkaline medium, the acidic properties of the hydroxyl groups of the fluonoid will appear and the dispersion will stabilize.
Yuri Mirgorod thank you for your response. I will make use of it.
- Badges
- Science topic