I've mistakenly put the cell that i wanted to freeze in the Room temperature overnight with 10%DMSO & 90%FBS. I wonder if the cell can still be alive?

28 May 2019 1 5K Report

Agnieszka Bojko

Few cells probably will be alive. But then you will have the clonal selection during thawing, therefore if I were you I would throw them away.

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