Culture Techniques

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs;... | Contact experts in Culture Techniques to get answers

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Questions related to Culture Techniques

How could I remove bacterial or yeast contamination from a growing cell line culture in t-75 flask?

I made thawing of a cryopreseved cell line, after transferring into t-75 flask and examination under microscope. it showed a contaminated cell culture. The contamination apparently had occurred...

04 April 2017 4,040 7 View

Marine microalgae turned yellow after 3 weeks. Why?

My algal culture turned yellow after 3 weeks of growing and I have tested the suspension for ammonium-N, nitrite-N and nitrate-N. Everything seems normal. The growth condition was set to...

04 April 2017 7,279 0 View

Can you identify Trichoderma harzianum in here?

I growed Trichoderma harzianum from a product using a medium with a "special" aditive. It growed in 3 to 4 days at room temperature 25ºC aprox, adn the medium was done with: 1000 ml water 10g...

04 April 2017 9,345 12 View

How does fungal contamination growth can be identified in tissue culture plate? and what we can do to stop its growth in culture and media?

my U87 cell are getting contamination that looks like as fungal. please suggest me how i can avoid fungal growth in my culture. how much Amphotericin B i can use in media if used to avoid fungal...

28 March 2017 3,252 1 View

Why the RPMI 1640 is recommended to be used as medium in Broth Microdilution assay?

What is the rationale behind this? In my case, I am going to use the assay for yeast cell, but I wonder that could I use YPD instead of RPMI 1640 as the medium?

20 March 2017 5,102 1 View

How may I get Fusarium free phytophthora?

During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...

09 March 2017 1,259 15 View

How does fungal contamination growth can be identified in tissue culture plate? and what we can do to stop its growth in culture and media?

my U87 cell are getting contamination that looks like as fungal. please suggest me how i can avoid fungal growth in my culture. how much Amphotericin B i can use in media if used to avoid fungal...

03 March 2017 7,102 1 View

How may I get Fusarium free phytophthora?

During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...

03 March 2017 8,941 16 View

Help me prevented contamination with HaCaT cells?

We was received some labolatories HaCaT cell. And Immediately, I went mycoplasma test performed. All were positive reaction(contamination). But Other kind of cells were not contaminated. If anyone...

03 March 2017 6,639 2 View

Has anyone hands-on experience with sebocyte culture?

I just want to know how easy or difficult to culture they are.Do I have to buy the manufacturers medium? Do I have to buy their specially coated flasks?How do I get the sebocytes to produce sebum?...

02 February 2017 9,489 3 View

Can i isolate protein from cryopreserved cell line?

Dear all, Suppose i have few cryopreserved cell lines. Without reviving the cell, will i be able to directly lyse the cell and isolate the protein from it? Thanks

25 January 2017 7,165 4 View

How to identify contamination in cell culture. Will the media change color for every type of contamination?

Greetings. I'm currently trying to culture B16F1 cells. But I observed a black spot/shadow in the culture flask. Hereby I attached two pictures from different culture flask, different batch and...

23 January 2017 6,164 1 View

Hello I'm working with BCG, can someone tell me the growing conditions in agar please? CO2, yes or no??

I tried to make a BCG colony count. I used Meddlebrook agar plates and my rpincipal doubt is if i should use an incubator with or without CO2. How long on averange BCG takes to grow?

19 January 2017 8,293 4 View

Can i isolate protein from cryopreserved cell line?

Dear all, Suppose i have few cryopreserved cell lines. Without reviving the cell, will i be able to directly lyse the cell and isolate the protein from it? Thanks

01 January 2017 364 5 View

Can anyone of you show me an image of SC (ATCC CRL-9855) cells to be sure about their welness?

I am working for the first time with SC cells ATCC CRL-9855, a suspension monocytes/macrophage cell culture. I don't undestand which are they conditions about viability and wellness. Can anyone...

01 January 2017 2,465 2 View

Hello I'm working with BCG, can someone tell me the growing conditions in agar please? CO2, yes or no??

I tried to make a BCG colony count. I used Meddlebrook agar plates and my rpincipal doubt is if i should use an incubator with or without CO2. How long on averange BCG takes to grow?

01 January 2017 4,352 5 View

How to differentiate dead cells and contamination?

i am using A549 cells its growing very fast and i am facing problem in differentiating cells and contamination but it is attending confluence very fast, i am using T25 flask. I can see dead cells,...

01 January 2017 8,775 5 View

Stationary phase at ~O.D. 2.0 with Terrific Broth?

Hey everyone, I am trying to do protein expression with BL21(DE3) cells and I am growing my cells in TB. I ordered the TB from RPI and added 4mL glycerol before autoclaving for 30 min. After...

21 December 2016 2,409 4 View

Incubator door was left ajar for probably 30min. Should I be worried about changes in behaviour/function of the cells that were being cultured there?

Observed some evaporation of the culture media...but inside of incubator felt mostly warm (i.e. 37deg).

14 December 2016 2,164 2 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

14 December 2016 9,588 3 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

12 December 2016 7,259 3 View

What is the best solvent for lysophosphatidic acid in tissue explnat or cell culture study other than chloroform:methanol:acetica cid ?

i am using buffalo endometrail explant tissue for LPA effect.

12 December 2016 6,030 1 View

How can I get "sulfate free" culture medium to incubate cells with sodium chlorate in order to abrogate heparan sulfate synthesis?

Hi, I trying to use sodium chlorate to abrogate heparan sulfate synthesis. The thing is that in many papers use "sulfate free medium", and they say that they buy it from SIGMA or GIBCO, but I...

12 December 2016 5,476 1 View

Incubator door was left ajar for probably 30min. Should I be worried about changes in behaviour/function of the cells that were being cultured there?

Observed some evaporation of the culture media...but inside of incubator felt mostly warm (i.e. 37deg).

12 December 2016 3,383 2 View

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