I growed Trichoderma harzianum from a product using a medium with a "special" aditive.
It growed in 3 to 4 days at room temperature 25ºC aprox, adn the medium was done with:
- 1000 ml water
- 10g agar agar
- 10g dextrose
- Clavulanic acid + amoxicilin (generously, amount not recorded)
- 0.01g of the product, that contains only T. harzianum: 10 8 ufc/g.
- And a drop of the aditive
I have been told that its not possible they are Trichoderma harzianum colonies: apparently they do not grow like that.
So I wonder if it would be possible for them to grow in a different way not yet seen, thank to that "additive" i am adding.
Can any of you identify Trichoderma harzianum in those Petri?
What interest could this have?
Thank you for your comments
I have never seen it growing like that. Which strain is it? Cultivation time and temp?
T.harzianum can grow on TSM Trichoderma Selective medium, Colony colours are different. so check on TSM
The medium is not PDA as I mentioned at the beginning
It was in 3 to 4 days at room temperature 25ºC aprox.
The medium:
- 1000 ml water
- 10g agar agar
- 10g dextrose
- Clavulanic acid + amoxicilin (generously, amount not redorded)
- 0.01g of the product, that contains only T. harzianum: 10 8 ufc/g.
- And a drop of the aditive
Got the details on a blog entry (SPANISH ONLY)
Thank you again
http://www.blog.naturaleza.eldietista.es/2012/02/cultivo-de-hongo-trichoderma-harzianum.html
Hi Yago
I am working on T. harzianum and I am never seen the colony like this,
ok, You are using a special media but from which reference or references
Sincerely
Thank you all.
Im sorry to say that Im not using any reference.
I just had an idea ... and I gave it a try.
I may need to recreate again the experiment and see if it works again. But it wont be easy, I would need to use different product (it shouldnt make a difference I guess) and get common antibiotics (which is at present more difficult than getting weapons or ilegal drugs).
It could be interesting but I still can´t see what could be the importance of this experiment.
morphologically these cultures do not look like Trichoderma
If you want to be sure make a slide observe under microscope that will clear all your doubt
Thank you all. I do not work in a laboratory neither have access to microscopes.
As most of you do, and this experiment growed in 3-5 days, I kindly ask you to try same experiment (without the additive) and see how different is.
As I used a product only containing T harzianum, I have no other choice to think it is.
But I cant investigate further considering that I do all this... at home:)
I don’t think so, as Mr. Sharma said, the colonies of Trichoderma are commonly green in color. Thank you
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