Culture Techniques

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs;... | Contact experts in Culture Techniques to get answers

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Questions related to Culture Techniques

How should I cut my buffer formalin fixed paraffin embedded scaffolds with cultured MSC's?

hello; I have a bad problem in cutting my samples. My scaffold is so dense; so baldly cut by microtom and cell layers that cultured on the top of scaffold separated from it.I could not have a good...

24 September 2016 299 9 View

Tryple, trypsin, trypsin in EDTA, EDTA or Accutase for SH SY5Y?

I am stuck with the dilemma of my SH SY5Y cells looking like they are clumping and growing on top of one another with some space around them. They are not spreading around very evenly like some of...

22 September 2016 2,032 2 View

I would like to culture innate lymphoid cells in vitro. Can anybody recommend a technique that works well?

I would specifically like to culture Type 3 Innate Lymphoid Cells

09 September 2016 2,370 2 View

Wharton Jelly Mesenchymal Stem Cell Recipe and Protocol? Anyone familiar?

Hi, i am growing Wharton Jelly Mesenchymal Stem Cell. Unfortunately, there is no attachment/proliferation occur after 2-3 days observation. 1.Is anyone here can share your experience handling this...

09 September 2016 1,799 9 View

Gene deletion problem in cloning?

First, i PCR amplyfied my gene (~ 2500bp) and then cloned it to the bacteria (DH5@). Second, i digested it with restriction enzymes (blunt end, ~1300bp) and left flanking sites (~1200bp) inside...

09 September 2016 3,284 2 View

How should I cut my buffer formalin fixed paraffin embedded scaffolds with cultured MSC's?

hello; I have a bad problem in cutting my samples. My scaffold is so dense; so baldly cut by microtom and cell layers that cultured on the top of scaffold separated from it.I could not have a good...

09 September 2016 7,576 10 View

What is the best concentration of PLL for adhering of Neural stem cells' s neurosphere?

I am using 1mg/ml concentration of PLL for adheration of Neurosphere , keeping it for over night at 37 degree in CO2 incubator but i m facing problem all the times. some time half of neurophere...

09 September 2016 6,479 3 View

We are getting black particles/ derbies in our cells. What could be the reason?

Media is fine. other things are also OK. Derbies are not present within the cells. They are not affecting cell's morphology or anything and mainly present in background. but its in large amount...

09 September 2016 8,312 4 View

Tryple, trypsin, trypsin in EDTA, EDTA or Accutase for SH SY5Y?

I am stuck with the dilemma of my SH SY5Y cells looking like they are clumping and growing on top of one another with some space around them. They are not spreading around very evenly like some of...

09 September 2016 1,873 2 View

Suitable media which one for liquid culture of Beauveria?

Dear all which media is the best one for liquid culture of Beauveria? I have Beauveria isolates and now I want to apply it on insects to test its performance. first time i used nutrient broth and...

09 September 2016 5,354 2 View

Does the contamination in one well of a 6-well culture plate affects other while changing media?

I am culturing an adherent cell in a 6-well plate. Unfortunately, one of the well got contaminated. Now, I need to change the media, and I just afraid while doing so the contamination get into...

09 September 2016 7,867 6 View

Stab culture medium preparation using LB agar?

I like to preserve some bacteria culture using stab culture technique but I like to know if I need to add more sodium chloride to my normal LB broth which contains 5g/L of sodium chloride

09 September 2016 5,122 4 View

Stab culture medium preparation using LB agar?

I like to preserve some bacteria culture using stab culture technique but I like to know if I need to add more sodium chloride to my normal LB broth which contains 5g/L of sodium chloride

01 September 2016 7,349 3 View

RAW cell culture and FACS?

Hi folks, I am growing RAW 264.7 cells in 12 well plates, RPMI with FBS and PS. When harvested and acquired cells in FACS, in few of the stimulated wells population is missing or second population...

24 August 2016 10,059 3 View

How can I prepare media containing alpha naphthol?

I would like to screen laccase producing fungi and I will use alpha naphthol. Please share me how can I prepare media that contain alpha naphthol as it is not soluble in water.

14 August 2016 6,599 1 View

Advice on slow growing MCF10A cells?

My students are having trouble with very slow growth of MCF10A's. Seeding at 1 x 106 cells per 75cm2 flask but not coming to confluency even after a week in culture. We are using exact Brugge lab...

12 August 2016 4,420 2 View

Streptomyces growth on cellophane?

Hi everyone, I'm trying to grow some Streptomyces on agar overlaid with cellophane, but haven't had any luck - no growth with S. coelicolor. This seems to be a pretty common Streptomyces...

12 August 2016 1,354 3 View

How long does the neuron in mouse DRG primary cell culture fully matured?

I'm trying to determine the optimal culturing time for my primary DRG cell culture, I wonder if anybody know how many days it takes mouse DRG neurons to be fully functionally matured from the...

12 August 2016 5,487 1 View

Marine bacteria for mussel

Hi everyone, I need to culture marine bacteria for mussel feeding, could anyone suggest me related to bacteria species? Thank you.

08 August 2016 7,716 3 View

How long does the neuron in mouse DRG primary cell culture fully matured?

I'm trying to determine the optimal culturing time for my primary DRG cell culture, I wonder if anybody know how many days it takes mouse DRG neurons to be fully functionally matured from the...

08 August 2016 5,288 1 View

As anyone ever had this Kind of contamination?

Hello, We have had a contamination problem with our primary cells. The media remains red, which is making me think that we could exclude bacteria. It takes at least one weak for that contamination...

08 August 2016 1,209 5 View

Any suggestions on growing Ins-1e cells as this is a new cell line for me?

I've had 2 cryovials given to me and I can't seem to get the cells to attach & grow. Complete media is 10% FBS, RPMI 1640 w/ L-glut, Sodium Pyruv, HEPES, Pen/Strep, & BME.

08 August 2016 6,475 0 View

Is it possible to to remove Agar from MS media, like by using Flitration?

I have ready made MS media with agar already included in it. so if i want to prepare liquid media is it possible to remove agar by any means.

08 August 2016 7,182 2 View

I constantly have floating cells in my SK-BR-3 culture. Is it normal?

Cells that adhered look healthy, but I am worried about the number of floating cells in my culture. Is it normal to have a significant number of floaters in SK-BR-3 culture? I am using RPMI 1640...

08 August 2016 10,021 5 View

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