I tried to make a BCG colony count.
I used Meddlebrook agar plates and my rpincipal doubt is if i should use an incubator with or without CO2.
How long on averange BCG takes to grow?
Hi Angela,
BCG grows better with 5% CO2, but you can incubate it without on Middelbrook plates.
Since it is a slow growing mycbacteria, it will take on average 2 to 3 weeks if subcultured from an aliquot. OR 4 weeks if cultured from clinical specimens or tissus.
Good luck
Hi Angela,
BCG grows better with 5% CO2, but you can incubate it without on Middelbrook plates.
Since it is a slow growing mycbacteria, it will take on average 2 to 3 weeks if subcultured from an aliquot. OR 4 weeks if cultured from clinical specimens or tissus.
Good luck
Hi Hafid,
many thanks for your reply.
I used an 5% CO2 incubator for my Middelbrook plates, but in many recent works it was not reported the use of this. I started from a pharmaceutical preparation, so I hope to see a growth around three weeks. Can I ask you to report me some bibliographical reference about this?
thanks you again for your kindness
I am grateful to you
Hi
For diagnostic purpose, we do not incubate Under CO2 conditions. and BCG grows well.
incubation of solid or liquid media Under 5 - 10 % CO2 results in an increase of yield and rate of growth.
I have sent you a pdf for our last publication.
You can see also this
https://www.researchgate.net/publication/5932602_Mycobacterium_bovis_Bacillus_Calmette-Guerin_Secreting_Active_Cathepsin_S_Stimulates_Expression_of_Mature_MHC_Class_II_Molecules_and_Antigen_Presentation_in_Human_Macrophages
Article Mycobacterium bovis Bacillus Calmette-Guerin Secreting Activ...
M. bovis BCG is a very slow growing mycobacterium as rightly said and aerophilic but grow better with 5% CO2 in media. Sometimes it takes upto 6weeks for it grows, pls be patient and extend the timing in case there is no any growth seen at 3-4weeks. Tnx
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