i am using buffalo endometrail explant tissue for LPA effect.
The sodium salt of lysophosphatidic acid is reasonably soluble in aqueous buffer such as PBS, with the solubility depending upon the acyl chain. For 1-oleoyl-phosphatidic acid sodium salt, the solubility is 8.3 mg/ml in PBS at pH 7.2.
https://www.caymanchem.com/pdfs/62215.pdf
Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...
11 December 2017 10,011 0 View
I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.
11 December 2017 9,186 0 View
I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...
11 December 2017 8,633 0 View
Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...
11 December 2017 6,112 0 View
During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...
11 December 2017 4,027 0 View
I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...
10 November 2017 7,023 0 View
I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.
10 November 2017 6,049 4 View
I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...
10 November 2017 3,132 3 View
Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...
06 July 2017 3,325 0 View
The pI of Histidine is about 7.5 which is almost equal to the physiological pH. So the total charge of Histidine will be neutral at physiological pH. Then how the His provides positive charge...
04 May 2017 5,899 2 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View
Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
24 February 2021 5,072 1 View