What is the best solvent for lysophosphatidic acid in tissue explnat or cell culture study other than chloroform:methanol:acetica cid ?

Perumal Nandhakumar @Perumal_Nandhakumar

12 December 2016 1 6K Report

i am using buffalo endometrail explant tissue for LPA effect.

The sodium salt of lysophosphatidic acid is reasonably soluble in aqueous buffer such as PBS, with the solubility depending upon the acyl chain. For 1-oleoyl-phosphatidic acid sodium salt, the solubility is 8.3 mg/ml in PBS at pH 7.2.

https://www.caymanchem.com/pdfs/62215.pdf

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