I just want to know how easy or difficult to culture they are.
Do I have to buy the manufacturers medium? Do I have to buy their specially coated flasks?
How do I get the sebocytes to produce sebum? Via transwell inserts?
(edited)
Benjamin
the attached articles will help you in this.
Thanks Saranya, I already know those papers. Have you hands-on experience with sebocytes?
Dear Benjamin,
Although your question was asked a while ago, our company provide sebocytes derived from hiPSC (please visit www.phenocell.com for more information).
Hope it helps,
Best regards,
Julien
I never used them, but we have them in our lab and i wanted to reuse them once they're out.
24 November 2015 6,792 4 View
I want do engineer a DNA-Microarray to analyse the virus-type of samples more quickly, is there any tool?
10 November 2015 5,030 0 View
10 November 2015 7,833 5 View
I found an old ÄKTA system at my new lab and want to use it, but there is no PC connected. To use it I'd like to spend no money. Is there any freeware to get it going?
26 October 2015 6,752 2 View
09 October 2015 8,486 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View