Culture Techniques

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs;... | Contact experts in Culture Techniques to get answers

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Questions related to Culture Techniques

Has anyone observed loss of global DNA methylation in HuLy or Tk6 cells without treatment - either due to wrong culturing or during DNA extraction?

I am working in cell culture using Tk6 cells and primary cells (human lymphocytes). Analysing the global DNA methylation with an ELISA I observed only 0.1-0.2 % of global methylation status....

01 January 2018 5,823 1 View

Cell culture media stock "contamination" by your won cells?

Hi all, I made a mistake for the first time during my 7 years of cell culture and I have no idea what I should do. So I just made a fresh cell culture media stock in the original bottle (Gibco...

10 July 2017 3,704 4 View

My Agrobacterium A136 is growing well in LB medium but not growing in AT minimal media? what can be the possible problem?

if A136 is growing well n LB it mean it is active, then why it is not growing in AT media? can anybody recommend some changes in At media specification?

07 July 2017 6,091 2 View

Please share how you treat Drosophila S2 cells!

- S2 Medium 10% heat-inactivated FBS + Pen/Strep then 0.2um-filter-sterilize? - I thawed a frozen S2 cells at P3, removed DMSO, and grew them about 2-3 days and split them 1:10 (so they are now...

07 July 2017 9,976 9 View

Contamination in cell cultures using anti-anti from GIBCO, anyone?

Has somebody experience cell culture contamination using media that contains 1X antibiotic-antimicotic from GIBCO. I have used this product for years, however, recently, I have many issues of...

07 July 2017 7,334 1 View

Cell culture media stock "contamination" by your won cells?

Hi all, I made a mistake for the first time during my 7 years of cell culture and I have no idea what I should do. So I just made a fresh cell culture media stock in the original bottle (Gibco...

07 July 2017 3,918 5 View

H2O2 decon for incubators without a diffuser

Does anyone know if I can sneakily use H2O2 to decontaminate an incubator that doesn't have an H2O2 diffuser? We have an old Sanyo tissue culture incubator, model MCO-18AIC, that has a fungal...

23 June 2017 2,713 1 View

Cell laboratory contamination

I'm working in an animal cell laboratory(on human cancer cells). it's about 2 weeks that all kinds of cells get infected and die a day after defreezing. I have disinfected the laboratory and...

19 June 2017 5,991 15 View

Mold contamination in my cell culture?

Hi, I have found something from the liquid nitrogen stock of a 293-derived cell line. Since the cell line is not replaceable, I am hoping to decontaminate my culture. It looks like mold to me, but...

06 June 2017 9,087 2 View

Zebrafish fin cell primary culture protocol

hi, I did zebrafish caudal fin cell primary culture. I want to discard remained caudal fin tissue when i do sub-culture. but it is difficult for me. how can i efficiently discard remained caudal...

06 June 2017 447 0 View

How to determine the biomass in a very dark-coloured cultivation media?

A part from OD measurement and FCM methods, do you know others trustable way to determine the biomass growth in media that have particles in suspension and precipitation and/or very dark...

06 June 2017 1,264 3 View

Cell laboratory contamination

I'm working in an animal cell laboratory(on human cancer cells). it's about 2 weeks that all kinds of cells get infected and die a day after defreezing. I have disinfected the laboratory and...

06 June 2017 2,584 16 View

How do suspension culture method affect on embryogenesis from callus of date palm?

Suspension culture method

31 May 2017 4,307 1 View

What are the minimum requirements for reporting Aflatoxicosis in Rats and why. Also can toxic levels be determined?

Culture techniques for the fungus Related research materials Toxin identification and related studies

19 May 2017 6,155 2 View

After a fish fin explant cells are radiating and growing up to few days. Then suddenly the monolayer starts shrinking and cells are dying. Plz help.

Hi friends!!! I am stuck with a fish fin explant culture. Each time I am trying an explant from fin tissue, cells will radiate nicely and monolayers can be seen. We found them growing for few days...

15 May 2017 10,001 2 View

Can anyone kindly provide some pictures about how the CHO cell culture looks like when it is contaminated?

I'm currently culturing the CHO cell in 125-mL shake flask. Some of the cultures had the trend that the viability keep decreasing. But I'm not sure about the reason, but it may be some...

05 May 2017 1,078 3 View

How to prevent fungal contamination in culturing flask of leishmania in field?

We are facing the problem of contamination despite of using vacuumed tubes, even antibiotics and anti fungal drugs were also used in the media (RPMI1640 supplemented with 10% FBS .

05 May 2017 909 2 View

After a fish fin explant cells are radiating and growing up to few days. Then suddenly the monolayer starts shrinking and cells are dying. Plz help.

Hi friends!!! I am stuck with a fish fin explant culture. Each time I am trying an explant from fin tissue, cells will radiate nicely and monolayers can be seen. We found them growing for few days...

05 May 2017 7,653 2 View

. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

In vitro culture technique.

05 May 2017 4,475 2 View

What's the role of pvp in in vitro culture technique?

Pvp added to white medium.

05 May 2017 8,502 0 View

Issues with cell contamination

Over the last couple of months, we have seen a contamination in our cell line (TR146) every couple of weeks (media is cloudy with white string like clumps), at one point there was a contamination...

24 April 2017 7,035 4 View

Axenic Chlorella protothecoides. How to get rid of fungi contamination?

We are growing C. protothecoides on two media: BB and TAP agar. However we have contamination with bacteria and fungi. We solved the problem with bacteria, with adding the antibiotics. However,...

04 April 2017 7,851 2 View

Issues with cell contamination

Over the last couple of months, we have seen a contamination in our cell line (TR146) every couple of weeks (media is cloudy with white string like clumps), at one point there was a contamination...

04 April 2017 2,708 5 View

Who has human norovirus VP1 plasmid?, if possible?

who has human norovirus VP1 plasmid?, if possible?

04 April 2017 6,318 1 View

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