During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free phytophthora?
Regards
Shuvrah
Hi Shuvrah,
See this reference 1
https://solutionsforyourlife.ufl.edu/hot_topics/pdfs/Tomato-Diseases.pdf
2
https://220.227.138.213/phytofura/downloads/PhytoFuRaAnnualReport2010-11.pdf
3
https://www.freshfromflorida.com/content/download/11398/144684/pp392.pdf
4
http://aciar.gov.au/files/mn-157/pdf/TECCIPM-14-(20Nov13)-fungal-fungallike-diseases.pdf
5
https://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n04_403.PDF
I follow the below medium to culture and the results are good.
How to prepare the Rye A agar medium
Formula∫ Rye A 120g/L
Calcium carbonate 0.02g/L
Tomato juice 150ml/L
Agar 17g/L
Amoxicillin 1g/L
Nystatin 0.01g/L
Rifampicin 0.01g/L
1. Wash Rye and then incubate in water over night, and do not close the lid over.
2. Add distilled water up to 1000 ml and boil it for 5 minutes and filter the water by gauze filter.
3. We must make the gauze filtrate should be one liter.
4. Wash tomato and cut into small pieces and grind in a mixer to make a smooth juice.
5. Take 1 liter T.J and add 15.02 g calcium carbonate and Boil the tomato juice for ten minutes and check the thickness.
Now take
1. Take 150 ml (150ml/L) tomato juice (tomato + CaCo3) and add into 2000 ml flask.
2. Add the 1000 ml of filtrate of the rye into the flask and mix well.
3. Add 17 grams of agar into the flask.
4. Add 1g amoxicillin + 0.01 g Rifampicin and sterilize it.
5. Put into Patty plates in laminar flow hood.
There are two good ways to do this: one, to use a selective medium like PARP, where only Phytophthora will grow (I have used this with great success in avoiding Fusarium). Two, Phytophthora will grow into the media, while Fusarium is more superficial. You can take a contaminated culture and cut out a cube of agar, then isolate from the *bottom*. I have also seen people melt a patch of plastic Petri dish out of the bottom of the plate and isolate from the bottom that way.
Hello Laith Al-Ani, Shehroon Khan and Nina Shishkoff Thank you all
Hi: This article is useful for you
https://academic.oup.com/mmy/article/53/6/603/2579417/Inhibitory-effects-of-antimicrobial-agents-against
- Badges
- Science topic