Suggestions for dyes to monitor bacterial growth kinetics?

I am planning to run an experiment that requires me to monitor growth kinetics in aerobic bacterial communities over approximately 7 days. The communities should have between 30-100 species...

17 June 2020 4,371 1 View

High Yields of Plasmid?

I am trying to produce and purify plasmids from competent cells for transfections however i keep attaining low quantities of around 80-120ng/ul. I am following a set protocol and purification...

28 October 2019 7,668 1 View

What are some dry materials that mimic human hypodermal tissue?

I'm looking for something that can sit out in dry air for a while, so a hydrogel is probably not the best solution. The closest I've found is trying platinum-cured silicones of different material...

26 September 2019 6,433 3 View

How do you combine multiple SEQ files iinto one file (FASTA or notepad)?

I use MEGA X for my phylogenetic analysis and sometimes I may work with 70+ sequences. If any of you know a method that can combine these sequences into one and free me from the ////tedious////...

31 July 2019 2,969 3 View

What AraBADC- strains do you recommend for membrane protein expression?

I am going to boldly attempt the purification of a viral membrane protein and want to try several expression systems to see what works best. One I would like to try is pBAD because (in my hands...

18 June 2019 3,825 1 View

What is the average number of compounds included in a QSAR study? Has anyone done statistics on this?

I'm asking because it seems to vary widely. Some authors claim to have done "SAR" with 4-10 compounds, which seems silly because the "relationship" between descriptors/structural features and...

25 July 2018 6,009 3 View

My Mooney Rivlin material keeps expanding rapidly on impact! Any solutions?

I am trying to model a hip with soft tissue impacting the ground using ANSYS 18 Explicit. From a paper, the soft tissue should be modelled as hyperelastic and it used the Mooney-Rivlin model. The...

18 April 2018 7,085 2 View

PCR - bands seen in water control but not in samples?

So this is a problem that has been inconsistent over the past year but has really become more prominent over the past few weeks - for practically every PCR I've run in order to genotype the mice...

22 February 2016 6,346 3 View

Does anyone know of a lung adenocarcinoma cell line that expresses both NKX2-1 & an alveolar marker such as CC10 or SP-A?

Cell line expressing NKX2-1 & an alveolar marker (CC10 or SP-A?)

22 July 2014 7,141 1 View

Does anyone know whether Sanjeev Arora's PTAS algorithm for the ETSP has been implemented into a practical tool?

In 1996 Sanjeev Arora published a polynomial time approximation scheme for the Euclidean Travelling Salesman Problem. I'd like to know whether anyone is aware of empirical results arising from an...

07 December 2013 4,207 1 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View