Hi,

I have found something from the liquid nitrogen stock of a 293-derived cell line. Since the cell line is not replaceable, I am hoping to decontaminate my culture.

It looks like mold to me, but I am not 100% sure. When they grow, the media is not changing colour. 

I have tried to add amphotericin B at a concentration of 2.5ug/mL to a few plates in which I did not see mold growth under microscope, but after one passage they appear again. 

Has anyone seen this kind of contamination before? What can I do to recover my cell line?

Thank you.

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