We are growing C. protothecoides on two media: BB and TAP agar. However we have contamination with bacteria and fungi. We solved the problem with bacteria, with adding the antibiotics. However, when it comes down to fungi we can´t get rid of it. We are currently trying to use anti-fungicid, called carbendazim. Yet this is not helping, as it seems that this fungi is resistant to it. We made stock solution of 0,3g/L in EtOH (100%), then put it in the media (after the autoclave) in the following concentrations: 0,05μg/mL, 0,1μg/mL and 0,5μg/mL. In every concentration the fungi grows well but there is no sign of algae whatsoever. From previous molecular analysis we think that the fungi might be Fusarium oxysporum.
Differential filtration can also be used to remove
yeast or other fungi from samples or crude cultures.
Fungal filaments are held well by filters, but their spores
usually pass through with the algae. To reduce or eliminate
fungal spores, add a little malt extract or other
organic enrichment to the filtrate. The fungal spores
will germinate in 1 or 2 days, forming filaments that can
then be collected on filters before they sporulate again
Please go to (Andersen 2005)
Dear Raid.
Thank you for the answer. However we did tried to grow them in rich media, but the fungi produced even more spores. Thus we abandoned to go further. If you have any other suggestions I would appreciate!
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