We can fused the protoplasts of 2 incompatible species via protoplast fusion, its mainly 2 types spontaneous and induced. First one spontaneous protoplast fusion by plasmodesmata of both cells. Induced protoplast fusion- we can induce it by 2 methods like 1. Electrofusion (electrophoresis) 2. Chemofusion by some chemicals or enzymes like DNA Ligase