If I want to clearly define the growth phase from a grwoth curve obtained from mammalian cell batch culture, what scale should I apply to the curve?

As mentioned above, I just conducted a batch culture of a mammalian cell line, and I got the daily viable cell density. But I'm a little bit confused about how to define its growth phase from its...

08 September 2017 6,726 0 View

Does CHO cell line considered as cancer cell line?

05 June 2017 8,091 1 View

Is there any criteria that what precentage of GC can be regarded as GC-rich in PCR primer design?

Are there any criteria that what percentage of GC can be regarded as GC-rich in PCR primer design?

05 June 2017 1,667 3 View

What is the proper format to list the describe the material used in article?

What is the proper format to list the describe the material used in article?

05 June 2017 350 0 View

Does disodium hydrogen phosphate anhydrous can be used to substitute for disodium hydrogen phosphate dodecahydrate when used for yeast medium?

As mentioned above

04 May 2017 2,054 2 View

About the 75% ethanol used in RNA extraction with Trizol, is 75% the volume precentage or weight percentage?

As what I describe in the question, I'm confused by this 75% ethanol. As I can see, in most of the protocol online, only 75% ethanol is mentioned, without stating if it's volume percentage or...

04 May 2017 8,103 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View