How to prevent fungal contamination in culturing flask of leishmania in field?

More Abid Ali's questions See All

What are good methods of synchronizing cancer cells in particularly in G1, S, G2/M stages ?

I am using MCF7 cell line which doubling time is greater than 30 hours, will the thymidine double blocks (block 1; 16 hr-->9 hr release-->block 2: 15 hr ) works better or serum starvation protocol?

04 May 2019 4,247 5 View

When we need to induce cells while doing a scratch assay?

I am working with inducible cell lines, I want to do scratch assay but I am not quite sure either to induce the cell a day before scratch assay so that we can clearly see the affect of our desire...

10 November 2018 4,136 4 View

Does a same DNA construct used for transfection of different cell lines, will affect protein size slightly or not?

I have transfected two different cell lines with same DNA construct but there is a slight difference in the size of the same protein harvested from those different cell lines. Did anyone...

09 October 2018 3,381 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Tomás Allende

What antiobiotics and antimycotics were added? There is overlap between some antimycotics and leishmania. Griseofulvin appears to be effective. Maybe this paper may be helpful.

Article Removal of bacterial and yeast contamination from Leishmania...

Abid Ali

thanks Allende, we are using penicillin G and Streptomycin sulphate