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Questions related to Culture Cells
Is there a method to detect trehalose in mammalian cell culture?
11 March 2018 5,409 3 View
We typically culture cells for 24-72 hours to obtain a population of exosomes, but how quickly after the cells are placed in culture do they start spitting exosomes out? 1 hr? 6hr? Has anyone...
03 March 2018 3,139 2 View
I am culturing cells in a microfluidic device for several days. Before the experiment, I am performing a Nitrogen cavitation procedure in order to expose the intracellular compartments of the...
03 March 2018 7,753 3 View
Hi everyone, Do you know a good method to isolate early or late endosomes for cultured cells ? I tried several protocols with sucrose gradient but i didn't see the interfases. I used 3 T175 per...
03 March 2018 4,657 2 View
I have a question for the culturing of MDA-MV-231. If I use complete L-15 (+10% FBS), can I cultivate the cells with 5% CO2? Because I saw that ATCC recommend the conditions without CO2 but it's...
03 March 2018 9,961 4 View
03 March 2018 3,222 4 View
I want to see VLP assembled in culture cells before purification under EM, is there a simple method to prepare the samples?
03 March 2018 7,488 0 View
recently I did experiment in culture cell treated with Curcumin. I made a stock of Curcumin with concentration 10 mM from 36 mg of Curcumin powder in 10 ml of DMSO. i treated my cell with...
03 March 2018 8,471 4 View
I am currently looking for (preferably hippocampus) culture/neurosphere/cell line with high proliferation rate, which are available to purchase in Canada.
13 February 2018 3,232 2 View
I am culturing these cells. I would like to compare what I have to others.
02 February 2018 1,324 0 View
We are planning to use testosterone on cultured cells. We tried to purchase some but a licence is required for the testosterone product. There are other products that don't require this licence,...
02 February 2018 3,695 2 View
02 February 2018 4,902 2 View
Hello, I am working on a project using different polymer films on a glass or plastic cover slip. I then culture cells on the film. I am having a lot of trouble keeping them from getting...
02 February 2018 3,278 0 View
I am not analytical chemist. However, I want to check for a possible new complex in cultured cells. Such as metabolically formed compound. The assumed molecular weight is not so high may be below...
02 February 2018 786 0 View
I'm culturing cells on PDMS microchannel. However, the PDMS loses its transparency due to cell media. I saw PDMS lose transparency in water, too. I want to prevent it because it hinders...
02 February 2018 3,523 9 View
I am researching into culturing cells from spiders any information will be appreciated.
23 January 2018 2,661 2 View
I am trying to do aniPOND to selectively capture my protein of interest associated at the replication fork in native condition. For this, I am labeling the replication fork with EdU followed by...
20 January 2018 825 1 View
Hellow. I'm doing deal with MC3T3-E1 osteoblast (ATCC) for the experiment. It has been quite a long time to culture this cell. However, something started getting strange. It was just very healthy...
01 January 2018 3,804 3 View
01 January 2018 4,279 1 View
I want to isolate the GFP labelled clone from the mixed population of U2OS cell line, because some cells loosed the GFP expression, but they are antibiotics resistance. I am trying with serial...
01 January 2018 9,874 3 View
01 January 2018 9,254 2 View
What is the best way to transfer cultured cells Between cities?
01 January 2018 1,271 4 View
want to study ceramide toxicity on cultured renal cells. Any special condition (serum-free medium, etc...) would be necessary?
01 January 2018 8,167 5 View
I am working with Adult Rat cardiac fibroblast and Adult mouse cardiac fibroblast. I use DMEM medium with 10% of FBS for culturing these cells. When I seed the Adult mouse cardiac fibroblast to...
12 December 2017 4,162 4 View