What are the potential prospects of combined chemotherapy in cancer treatment??

Recently, researchers have been looking for a optimum combination of chemicals to boost up the anticancer potential of certain chemical compounds. May be to overcome the resistance to of some...

01 February 2018 8,508 3 View

How can I use Alpha lipoic acid in DMEM medium?

I have been trying to use Alpha lipoic acid (ALA) in DMEM for my experiment. I dissolved ALA in ethanol. However, When I put ALA in DMEM, it immediately produce clot. Does anyone face same problem...

09 October 2017 5,895 0 View

Could anyone please suggest me, How can I dissolve Iron (Fe) powder in water/other solvents??

Fe (powder) purity 98%

02 March 2016 6,178 8 View

Is Lactate Dehydrogenase (LDH) activity in cell culture supernatant an indicator for possible necrosis?

01 January 1970 4,100 4 View

Is it possible to explain cell membrane status using trypanTrypan blue staining method???

Specifically, for study viable/dead cell it is ok but what about the other????

01 January 1970 7,816 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View