Has anyone, ever tried to culture primary cells from spiders?

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Call for paper on special issue at WATER-MDPI, Interested?

Dear Colleagues, I am pleased to inform you that a Special Issue of the WATER-MDPI journal on the topic of "Advances and Challenges in Hydro-Climatological Modeling and Uncertainty Analysis" is...

21 February 2024 3,637 1 View

Change unit from mV to mAU on HPLC?

Dear I have PerkinElmer instrument. I am facing a Problem. I am not understanding how i can change unit from "mV to mAU" on software. so that my plot can appear mAU versus concentration. Can...

29 August 2023 7,129 2 View

Quantification of citric acid on HPLC?

Dear , i am trying to run my sample. but my chromatography is straight line for my sample. i am trying to make a standard curve for this, i am making standard solution of of citric acid having...

15 August 2023 4,826 0 View

If peaks comes on HPLC chromatography either i run blank or sample?

i am new in HPLC running. while running HPLC i am watching same peak patter when i run blank or i run sample. please guide me how i can solve this issue.

14 August 2023 831 8 View

I am facing an issue with the polyacrylamide gel for western blot. What could be the reason?

Hello, I am facing an issue with my polyacrylamide gel for western blot. This is 12% gel and I have used precast gel and made it with the recipe provided by BIO-RAD. I have been using the same...

14 August 2023 158 3 View

Quantification of citric acid on HPLC?

can anyone guide me for a Quantification of citric acid on HPLC? firstly i am facing a problem my HPLC baseline is not getting zero. On blank sample like methanol it is showing strong peak.

10 August 2023 5,747 5 View

Are there problems in biological ammonia removal for secondary effluent wastewater?

After had been removed BOD and TSS, the amonia removal is required biologically. Are there problems to growing nitrifying bacteria for nitrification and difficult to settle it in sedimentation tank?

26 July 2023 2,362 4 View

Ways to Enhance citric acid production?

Dear i am trying to produce citric acid from sucrose using aspergillus niger strain? can anyone guide me in this step I should add sugar at different days of fermentation or i should add complete...

25 February 2023 2,748 0 View

Recovery of citric acid from fermentation broth?

can anyone help me in the recovery of citric acid from fermentation broth I want to recover citric acid using Ca(OH)2 when add Ca(OH)2 in it. how much Ca(OH)2 i should add to get pure calcium...

30 January 2023 7,476 2 View

0.1% phosphoric acid solution?

for the HPLC Analysis, i want to use 0.1% phosphoric acid as a mobile phase. can anyone guide me how i can make 0.1% phosphoric acid solution to make a standard for citric acid quantification.

26 January 2023 2,259 4 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View