Does anyone have good protocol for dissociation of hippocampal neurons from adult mouse (flow cytometry/RNA seq)?

I am looking for any good protocol for dissociation of single hippocampal neurons from adult mice. We'll perform flow cytometry and eventually work on qPCR. As our lab is not specialized for...

06 July 2019 8,298 2 View

Are there any reliable antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus?

I have Camk2a-Cre mice model, and would like to have antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus CA1 regions. Do you know any good antibodies for...

10 November 2018 9,467 4 View

Do you have any suggestions for neurosphere assay protocol, using adult mice?

Do you have any suggestions for growth factors (EGF and FGF) effective/useful for proliferation medium? How do you sterilize surgical equipments - autoclave or buffer? Any tips to share -...

01 February 2018 1,114 2 View

Problems in using mouse host primary antibodies on mouse brain sample (IF/free floating staining).

I am doing free floating immunofluorescent staining on my mouse brain sample. I've been having difficulty using rodent host (ie. mouse, rat, guinea pig) primary antibodies, and I don't know why....

01 January 1970 6,925 13 View

Having difficulty IF staining on 5XFAD mouse brain sample (free floating).

I am not sure if we are supposed to change staining protocol for different mouse strains, but I am having difficulty staining (free floating immunofluorescent staining) on my 5XFAD mouse brain...

01 January 1970 3,227 5 View

Are there any rodent behavioural experiments that can substitute contextual fear conditioning test?

I am investigating the role of histone modification during hippocampal memory formation. It is the best if I could perform contextual fear conditioning on mice. However, we unfortunately do not...

01 January 1970 9,449 1 View

Reversal learning deficit, but normal memory extinction on Morris water maze test

I am wondering if anyone has seen the results of morris water maze like this; - normal acquisition (decrease in escape latency) BUT - significant deficit in reversal learning - normal memory...

01 January 1970 8,033 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View