How functional study effect when gene is fluorescently tagged endogenously by CRISPR knock-in or when it is over-expressed?

If I am studying the gene which is tagged by fluorescent tag endogenously by CRISPR/Cas9 Knock-in or if the same gene over-expressed and tagged with same fluorescent tag. Then what difference it...

05 June 2018 9,353 3 View

Whether Knock-in in hypertriploid U2-OS cell line affect insertion through CRISPR technology ?

I am planning for Knock-in in U2-OS cell line, which is chromosomally highly altered, with chromosome counts in the hypertriploid range. Hence whether the insertion of new gene into it cause any...

11 December 2017 8,607 2 View

How to transfect mCherry gene into the promoter of MDC1 gene by using CRISPR/CAS9 technology?

Hello Everyone, I want to generate transfected U2OS cell line , in which MDC1 will be fused with mCherry at its endogenous locus so mCherry will be under MDC1 promoter. How can I generate this...

10 November 2017 8,782 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View