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Questions related to Culture Cells
If so which vehicle and what concentration in mammalian cell culture setting. I am currently adding unsaturated FFA, comparing with EtOH and with Albumin, around 10- 100uM.
04 April 2017 10,026 0 View
I'm looking for the simplest protocols to induce oxidative stress in several cell lines but I need to avoid a protocol that induces LDL oxidation. Thank you in advance!
04 April 2017 4,093 4 View
I'm trying to grow a confluent layer of epithelial cells on a filter, then would like to disperse the layer off the membrane. I don't want individual cells to disperse, I want the cell-cell...
03 March 2017 8,823 1 View
I'm trying to cultivate a microalgae culture in glass or plastic recipients to obtain hydrogen, but before this the medium must be deprivated of sulfur. Thanks!
03 March 2017 5,407 0 View
We have to use a non-enzymatic cell dissociation to detach cells and EDTA/versene is taking far too long. What concentration of sodium citrate is usually recommended to detach cells?
03 March 2017 4,255 1 View
Please, I need isolate exosomes derived from B16F10 cells. Does a medium without FBS
03 March 2017 300 1 View
Hello! Is anybody using nonyl acridine for staining mitochondria and measuring it with a plate reader? Could you help with the protocol? What concentration of NAO do you use? Do you load the dye...
03 March 2017 7,410 0 View
I am working with human adipocytes. The differentiated adipocytes are full of lipids and therefore float in standard cell culture medium. I think that decreasing density of medium would prevent...
03 March 2017 5,637 4 View
I need to protocol or kit that can evaluate one gene expression on cell suspension treated with oligonucleotides in 96 well plates.
03 March 2017 8,074 3 View
I want to grow these cells for stimulating with different cytokines and see their effect on growth.
02 February 2017 9,287 1 View
Do the cells look not attached to the surface of flask? I have initiated MCF7 cell culture 2 days ago. I have used DMEM/f-12 supplemented with 10% FBS, 1% MEM non-essential amino acids, 10 ug/ml...
02 February 2017 2,493 5 View
MA104 cells are not growing well when recently thaw. I´m using MEM media with phenol red buffered with sodium bicarbonate. Media osmolality is 296 mOsm/kg, pH is 7.56 both values were measured...
02 February 2017 8,607 0 View
I've been trying to assess fluorescein leakage from isolated cells plated on poly-l-lysine dishes by using fluorescein diacetate as a vehicle; however, I've noticed that shortly after I load...
02 February 2017 9,865 4 View
Hello everyone, I am working with 3t3-l1 cell and I have been having a problem with peeling and curling effect when differentiated. My cells start to peel off and curl one or two days after...
02 February 2017 4,949 5 View
I am trying to measure proliferation of PBMcs by flow cytometry. Does anybody have a good protocol to stimulate PBMCs with PHA and measure proliferation using the EdU Iclick kit? How long can the...
02 February 2017 5,116 1 View
I am using HepG2 cells as the cell model. I am giving treatment of estradiol. But i want to be sure that my estradiol drug is dissolved correctly. so anyone can suggest me a known E2 inducible...
01 January 2017 2,376 0 View
I am using HepG2 cells as the model system
01 January 2017 9,654 1 View
I do culture the cells till confluent. then make the scratch and wash 2 times with media. But still there are many floating cells in the wound. Is there any special care or way to remove those...
01 January 2017 6,507 3 View
i want to put MTS on suspended cells to evaluate viability but the data sheet demonstrate the details for attached cells but not demonstrate the fine detalis for suspended cells and there is some...
01 January 2017 8,073 2 View
I want to try a long term culture of Lens Epithelial cells, culturing them for more than two months.Up until now i have been culturing the cells for 3 to 4 weeks in RPMI culture medium...
01 January 2017 4,940 3 View
I am studying about relations between warburg effect and cancer evolution and find this question. Why do unicellular organisms and cultured cells use aerobic glycolysis?
01 January 2017 7,157 0 View
it's a surprising finding. when I added Trizol to fully differentiated adipocytes culture to extract RNA, white adipocytes all floated while beige adipocytes remains attached on the culture plate.
12 December 2016 3,014 2 View
Hello, I am checking the anti- proliferative property of curcumin on cultured cells. I have docked my molecule with few receptors using discovery studio. I am not able to analyse my results. Can...
12 December 2016 4,545 0 View
Am evaluating the the oxidative stress-inducing effect of a compound on cultured adherent cells and would like to measure ratio of GSH/GSSG as marker of oxidative stress
11 November 2016 3,487 2 View