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Questions related to Cell Biology
I am looking at the interactions between a ligand and a protein. I have set parameters in order to rule out 'irrelevant' interactions and these parameters have set values for distances and angles....
05 April 2021 6,500 0 View
Hello everybody, i hope somebody can help me with my cells. Since 2 weeks they started to change morphologically, as you can see in the two pictures with the smaller magnification (they started...
19 November 2020 1,410 3 View
I have read many articles where researchers get RVG-Exo by transfecting the exosome producing cell through RVG Plasmid. I wanted to ask if anybody knows, can we decorate exosome with RVG directly...
21 October 2020 2,481 2 View
A super basic cell biology question for cell biology people. I will try to measure protein phosphorylation, and I will do western blot to see whether the phosphorylation occurs. If phosphorylation...
08 June 2020 9,128 4 View
HI, I'm studying endocytosis pathway of certain particles, using Dynasore and MbCD endocytosis inhibitors. My question is, that whether dynasore could inhibit both clathrin-ME and...
09 May 2020 9,490 1 View
I am curious about two specific things: - Why do pseudounipolar neurons have one axon (as opposed to a dendrite + axon like multipolar neurons)? How does this structure reflect sensory function? -...
14 April 2020 4,568 4 View
Hi, I have stumbled upon an article about translocation of AKT into mitochondrial matrix upon IGF stimulation and as I tried to find some mechanism of this transport I found another articles...
25 March 2020 1,108 2 View
Dear colleagues, I've been working on some lipid extraction analysis with QDa HPLC. I start with some biological samples that are spiked with a different final concentrations of a lipid mix...
15 March 2020 1,050 4 View
Some diagrams show that they are different. However, some explanations say that they are the same. Is it different among species? If it is, why should there be a Dicer in the RISC, or that a Dicer...
28 February 2020 3,045 3 View
I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I...
25 February 2020 7,174 2 View
02 February 2020 8,106 2 View
I was reading into granulocytes and it came to my attention that since they are essentially digestive granules they might be analogous to lysosomes. I was wondering whether this was the case but...
02 February 2020 5,656 3 View
02 February 2020 4,035 3 View
We have been culturing MSCs from knee bone chips in which the MSCs migrate out into the culture flask. These have been taken from someone else in our group and resurrected from frozen (P3-4)....
08 January 2020 8,253 3 View
01 January 2020 4,903 3 View
Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...
01 January 2020 1,186 2 View
I am trying to Biotinylate the cell surface proteins of mouse neutrophils before pulling them down with Streptavidin-magnetic beads...
01 January 2020 8,738 2 View
Is there any portal or application that accepts Seurat objects as input and lets me see genes’ expression across the clusters? Of course, my lab’s service provider can help using Seurat itself,...
12 December 2019 2,365 4 View
Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...
14 November 2019 5,375 3 View
11 November 2019 2,470 3 View
Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...
14 October 2019 6,001 6 View
10 October 2019 7,130 7 View
I found several collapsin (formerly called semaphorin) response mediator proteins (CRMP) upregulated in a proteome of our primary hippocampal neuronal culture-treated with neurotrophin-mimetics....
08 August 2019 4,224 2 View
Hi everybody! I´m trying to improve my protocol for CD4 T-cell expansion isolated from murine spleen and lymphnodes. Do you have any suggestion? Thank you a lot!
24 July 2019 3,701 7 View