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Questions related to Culture Cells
I'm going to do a wound healing assay with a cytosine arabinoside like proliferation inhibitor, but I don't know if I have to administrate this inhibitor every 24 hours o what time is the best....
12 December 2017 1,054 2 View
I am working with primary cell culture at the moment and we have had issues with RBC contamination for the purposes of visualization of our cultured cells. Additionally, I've noticed pH changes...
12 December 2017 3,597 4 View
05 December 2017 3,535 3 View
I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...
11 November 2017 5,702 3 View
As above and i'm not sure why do the cells not remain in 3D aggregates. I tried to mix 2 cell types together and they do not "stick". What are the possible reasons for this? Thank you.
11 November 2017 1,513 2 View
07 November 2017 5,618 3 View
Hi All, I am trying to do a 3D cell culture for MSC cells and condrocyte Cell lines for cartilage application using different concentration of photocrosslinking alginate and/ or gelatin...
27 October 2017 1,034 8 View
I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...
11 October 2017 2,799 2 View
Hello All, I am trying to do a 3D cell culture for Smooth Muscle cells and Vascular Endothelial Cell lines. I found a lot of reviews and publications but I was wondering if anyone can refer me...
11 October 2017 7,459 5 View
10 October 2017 888 8 View
In the past few days I found a structure that looks like cell sheet in my culture (the cells are MDA Pca 2b). It covers the surface of the flask, but it tears appart when I wash the surface with...
10 October 2017 9,514 0 View
We are trying to grow the U251 and U87 cells in Neurosphers. At first when I cultured the cells in DMEM high glucose with 1% PEST, 1% L-Glut and 1% NEAA with 10% regular FBS (non exosome...
10 October 2017 2,771 0 View
Hi For the CD8+ CD28- T cell expansion ( terminally differentiated memory T cells), in addition to the anti-cd3, what cytokines or other substances should i add?How about IL-2, IL-15, T+I or IL-7...
10 October 2017 9,933 4 View
Hello, I am planning to enrich O-GlucNAc proteins in Jurkat T-cells by metabolic labeling before performing drug-treatment with Cisplatin. I have some practical questions regarding this: 1. Is it...
10 October 2017 7,336 0 View
I use Polyacrylamide gels of various stiffness in my study. To culture cells on PAA gel, we functionalize it with Sulpho-SANPAH followed by treatment with collagen type-1. I want to see the effect...
10 October 2017 3,445 0 View
Hi, I am trying to polarize human monocyte derived macrophages (M0 cells cultured with medium only for 5 days) by IFNg and IL-4. The methods from papers are always for 48h polarization. I am...
10 October 2017 3,683 5 View
10 October 2017 1,503 5 View
I will isolate mouse naive T cells and, prior to activation, I would like to KO a doubled floxed gene. Has anyone ever tried this, in T cells or in another suspension primary culture...
10 October 2017 7,774 0 View
Just thawed a vial of Kasumi-1, and another of KG-1 AML AMLcell line. Does anyone have experience with ~about how long it takes for these to go through cycle?
29 September 2017 404 4 View
Microgravity isn't similar to our body, is it?
22 September 2017 899 2 View
09 September 2017 7,713 2 View
If anyone has tried this before and has a protocol I really appreciated. thanks
09 September 2017 2,411 0 View
Hi everyone, I am trying to measure intracellular iron content from cultured cells using the colorimetry method (chromogen reagent), however so far unsuccessful. The absorbance readings at 540 nm...
09 September 2017 4,079 1 View
The cultured cells are suspensions and are not uniformly distributed in the culture medium at all.
09 September 2017 3,919 3 View