What are the acceptable biological and technical replicates in realtime RT-qPCR?

What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...

07 August 2018 298 5 View

How can I study cells that can attach to Petri dishes and grow very well?

I am culturing one kind of cells with some stem cells criteria. While I was preparing embryoid bodies in Petri dishes from these cells, they were attached to the bottom and grow naturally and now...

10 November 2017 2,169 3 View

What is the lethal heat for cell lines?

09 October 2017 2,061 5 View

What is the specificity of ELISA in evaluating melatonin from different sources or different species?

Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?

07 August 2016 7,423 5 View

Is there any non-invasive (non-surgical) method for early mouse embryos retrieval ?

10 November 2015 142 7 View

What is the scientific importance of chimera/chimeric animals?

Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...

10 November 2014 6,134 11 View

The case of Shigeaki Kato, is not worrying for the future of science?

This week another three papers have been retracted from Nature Cell Biology journal authored by S. Kato. Until now more than 25 papers have been retracted from highly prestigious journals...

10 November 2014 1,187 14 View

Can I isolate RNA and miRNA from a single cell by heating at 95 C?

I want to isolate RNA and miRNA from a single cell. I read the work of Dr Wangh L.J. laboratory and I know some commercial kits but I need a cheap/easy method. I read an abstract from Applied...

11 December 2013 4,220 3 View

Can I visualize the microvesicles or the exosomes by confocal or phase contrast microscopes?

Microvesicles (MVs) or exosomes measured 30-1000 nm and all publications noted them through electron microscope but unfortunately EM is unavailable in our institute. Is it possible to visualize...

06 July 2013 3,622 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View