03 March 2018 4 3K Report

Is there a method to detect trehalose in mammalian cell culture?

More Islam Saadeldin's questions See All
What are the acceptable biological and technical replicates in realtime RT-qPCR?

What is the minimum number for biological and technical replicates for a reliable results of RT-qPCR? what if I collect/gather all the biological samples from different replicates of the same...

07 August 2018 459 5 View

How can I study cells that can attach to Petri dishes and grow very well?

I am culturing one kind of cells with some stem cells criteria. While I was preparing embryoid bodies in Petri dishes from these cells, they were attached to the bottom and grow naturally and now...

10 November 2017 2,315 3 View

What is the lethal heat for cell lines?

I noticed in publication that lethal heat for cell line is 45 C for 60 min [Cell Stress & Chaperones (2001) 6 (4), 326–336]. Is this degree considered lethal for all cell lines? Is there any...

09 October 2017 2,183 5 View

What is the specificity of ELISA in evaluating melatonin from different sources or different species?

Melatonin is derived from amino acid L-tryptophan. Is there any difference between ELISA specificity to the endogenous (naturally occurring) and the exogenous (drug or implants) melatonin?

07 August 2016 7,558 5 View

Is there any non-invasive (non-surgical) method for early mouse embryos retrieval ?

I need a non-surgical method for early mouse embryos recovery (morula or blastocyst stage) such as uterine lavage. 

10 November 2015 259 7 View

What is the scientific importance of chimera/chimeric animals?

Chimeric embryo and/or animals can be generated through injection of pluripotent cells in the early stage developing embryos (Morula or blastocyst) or through aggregation of different kinds of...

10 November 2014 6,256 11 View

The case of Shigeaki Kato, is not worrying for the future of science?

This week another three papers have been retracted from Nature Cell Biology journal authored by S. Kato. Until now more than 25 papers have been retracted from highly prestigious journals...

10 November 2014 1,347 14 View

Can I isolate RNA and miRNA from a single cell by heating at 95 C?

I want to isolate RNA and miRNA from a single cell. I read the work of Dr Wangh L.J. laboratory and I know some commercial kits but I need a cheap/easy method. I read an abstract from Applied...

11 December 2013 4,358 3 View

Can I visualize the microvesicles or the exosomes by confocal or phase contrast microscopes?

Microvesicles (MVs) or exosomes measured 30-1000 nm and all publications noted them through electron microscope but unfortunately EM is unavailable in our institute. Is it possible to visualize...

06 July 2013 3,770 9 View

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

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