want to study ceramide toxicity on cultured renal cells. Any special condition (serum-free medium, etc...) would be necessary?
You can buy various ceramides such as acetylceramide from Sigma-Aldrich.
Hello Istvan,
in my lab, we order ceramides from Avanti Polar Lipids, they offer a broard range of different ceramides. You could also check this publication:
Article Antibacterial activity of ceramide and ceramide analogs agai...
There are also results for cell cytotoxicity.
There are multiple good providers, in th end it will depend on what kind of CER you will be using. Avanti polar lipids as far as i'am aware offers the broadest spectrum of ceramides but is also quite expensive.
Certain fatty acids and sphingoid bases found at mucosal surfaces are known to have antibacterial activity and are thought to play a more direct role in innate immunity against bacterial infections. Herein, we analysed the antibacterial activity of sphingolipids, including the sphingoid base sphingosine as well as short-chain C6 and long-chain C16-ceramides and azido-functionalized ceramide analogs against pathogenic Neisseriae. Determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) demonstrated that short-chain ceramides and a ω-azido-functionalized C6-ceramide were active against Neisseria meningitidis and N. gonorrhoeae, whereas they were inactive against Escherichia coli and Staphylococcus aureus. Kinetic assays showed that killing of N. meningitidis occurred within 2 h with ω-azido-C6-ceramide at 1 X the MIC. Of note, at a bactericidal concentration, ω-azido-C6-ceramide had no significant toxic effect on host cells. Moreover, lipid uptake and localization was studied by flow cytometry and confocal laser scanning microscopy (CLSM) and revealed a rapid uptake by bacteria within 5 min. CLSM and super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy demonstrated homogeneous distribution of ceramide analogs in the bacterial membrane. Taken together, these data demonstrate the potent bactericidal activity of sphingosine and synthetic short-chain ceramide analogs against pathogenic Neisseriae.
References
V Manju, N Nalini. Effect of ginger on lipid peroxidation and antioxidant status in 1,2- dimethyl hydrazine induced experimental colon carcinog-enesis. J Biochem Tech (2010) 2(2):161-167 ISSN: 0974-2328
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