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Questions related to Culture Cells
I plan to do a cytotoxicity assay on cultured cells. I would like to include purified exosomes. I usually resuspend my exosome pellet in PBS, however, would it be possible to resuspend the exosome...
08 August 2017 10,009 1 View
Hello everyone, I want to measure the expression level of my gene of interest though qPCR analysis upon knocking it down by RNAi interference in cultured cells. As far as I understood, the basic...
08 August 2017 2,909 5 View
Hi I'm looking for the best way to add rotenone to the media of cultured cells.
08 August 2017 10,123 2 View
I am culturing cells and want a quick way to routinely record their morphology for future reference. At the moment, we use a Nikon Eclipse Ts100 to observe the cells while culturing, but it only...
08 August 2017 9,650 0 View
We are working with Caco-2 cells in culture and we need a protocol to measure total glutathione and also superoxide anion production. Can someone give me the protocol? Thank you very much!
08 August 2017 8,889 1 View
I am doing whole cell patch clamp electrophysiology on acute cultures of midbrain dopaminergic cells. I normally start doing my experiments on DIV 7 and go as far as DIV 12. However, I have...
08 August 2017 7,271 6 View
How can I degrade dna extract? I used H2O2 with concentrations ranging from 50 mM to 500 mM but the band on gel only appeared thinner than control but not smeared, i used U.V lamp and still had...
08 August 2017 6,707 5 View
I looked the literature and found a couple of nice papers for the method, but they are all on culture cells assessment. I was wondering if someone ever read something on in vivo quantification and...
08 August 2017 5,481 0 View
I have z problem. I need to remove albumin from serum to detect another protein. Can anyone recommend a KIT? Thank You, sincerely, Gośka.
21 July 2017 4,032 3 View
We are applying ultrasound for live cells grown in multi-well dishes. There is no a noticeable effect on cell viability. How to show that there is an effect of ultrasound at all in the cell medium?
21 July 2017 3,643 2 View
I thawed A20 cells and I had around 70% mortality, because there weren't freeze properly, but that's all I have to work with. I'm cultivating them in RPMI media supplemented with 10% FBS, 1%...
10 July 2017 5,646 5 View
I am treating 293T cells with conditioned media and measuring DNA damage using yH2A.X staining. I've tried using Raw Integrated Density to quantify the damage, but am not sure if this is the best...
07 July 2017 8,703 2 View
07 July 2017 8,593 2 View
07 July 2017 6,692 5 View
Hello all. I am trying to grow THP1 cell line with medium frer of exosomes. Therefore, I am looking to see if there is any way to culture THP1 cells without FCS (or FBS) that is known to contain...
07 July 2017 6,790 2 View
07 July 2017 4,258 4 View
Hi everyone, I am finding all available techniques to observed cultured cell if the substrate is not transparent. Is it possible to get fluorescent images? Could you give me some suggestions for...
07 July 2017 2,177 0 View
Hello! I would love to know about the production of secondary compounds, especially about phenolic compounds which are produced in the process of culturing plant tissue. It is supposed to be...
28 June 2017 8,116 10 View
Hi, I will be receiving soft mass tumor samples from needle biopsies in order to study single cell mechanics. However, we are working with a very small sample (~1000 cells). I am not sure if it is...
08 June 2017 1,201 2 View
I have done IHC before, but only with tissue samples. I was wondering if the protocol is same for tissues and cultured cells and what precautions I would need to take, while staining monolayer cells.
06 June 2017 1,864 4 View
I use LS 180 cell line that already trypsinized. I read in ATCC, LS 180 have never been trypsinized. so after that, I never use trypsin but my cells easily detach now. What should I do?
06 June 2017 4,198 1 View
06 June 2017 393 2 View
I just got VERO C1008 (Kidney; African Green Monkey) and BHK-21 (C-13) (Kidney; Syrian Hamster) cell lines from ATCC. Tried VERO cell with DMEM+10%FBS+P/S first and it grew much slower compare to...
06 June 2017 9,433 6 View
I intend to culture cells in a 3D matrix, and retrieve cells at certain timepoints for downstream phenotyping (by flow cytometry). I heard that the process of retrieving cells from a gel matrix...
06 June 2017 2,023 0 View