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Questions related to Culture Cells
We have cultured cells on PDMS functionalized with a Matrigel/Collagen mixture, and are wondering if new cells can be directly reseeded after enzymatically removing the original cells. We aren't...
29 November 2018 2,287 3 View
Hi, Can someone suggest me a method of fixing cultured neurons for fluorescent staining and long term storage? Would I not need to go for paraffin fixing since they are cultured cells?
21 November 2018 9,355 3 View
How to measuring extracellular ATP level in tumor specimens, not the culture cell? Is there are commecial Kits available?
18 November 2018 1,417 1 View
I want to stain an intracellular protein in 4% FA fixed cultured cells. I have to permeabilise the cells. Triton is to harsh (The ultra structure is too much damaged for EM). I need a milder...
11 November 2018 7,309 5 View
11 November 2018 3,806 1 View
I am working in with embryonic rat cardiomyocytes and I have in immunolabelling connexin proteins in the cultured cells. Right now I would like to check for the presence of gap junction proteins...
11 November 2018 6,975 0 View
Does anyone have any experience with the Petaka G3 system for culturing cells under hypoxic conditions? Although the system is around for several years, I am not able to find a single publication...
11 November 2018 3,241 3 View
How can I detect pyroptosis in human mo-DCs induced by a pathogen? Are there some comercial kit?
11 November 2018 5,342 0 View
Would culturing cells in sterile dishes be better to maintain pH?
26 October 2018 9,694 3 View
I am a master student works with biomaterial, since I want to get some in vitro studying results for my new type of nanofiber scaffold, I has tried 3 times to culture HUVECs cells. But at first...
10 October 2018 6,255 3 View
10 October 2018 5,196 3 View
I want to detect signals of death in a insect tissue, it is possible do that with propidium iodide, I have seen that people used iodide with culture cells.
22 September 2018 9,909 3 View
09 September 2018 8,948 4 View
Hello everyone, I am currently make a stock and providing research samples in my workplace, I would like to ask your experties what are the best culture media you used to isolate healthy and...
09 September 2018 4,591 3 View
Would anyone has experience culturing Huh7 cell line please help me. I first started to culture the Huh7.5.1 cell line about 3 months ago, and I noticed the cells started to grow on top of each...
09 September 2018 4,244 1 View
Hallo I need to know if HepG2 or Huh7 or IHH cells produce bile acids. Have you never tested it? thanks maria
09 September 2018 3,904 1 View
Hello It's unclear to me the concept of IVCC(integral viable cell concentration) and I'm struggling to calculate them. I have sampled cultured cell in day1 , day3, day5, and day7, and their viable...
09 September 2018 3,347 3 View
Hi I have noticed one thing in cell culture is that the cells love to attach themselves at the side of the the culture vessels more than at any other part of the flask. Also, the these cells...
28 August 2018 1,936 4 View
How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...
08 August 2018 8,418 0 View
I have read many user manuals but I could'nt find some specific data for cells in suspension as EBV immortalized B lymphocytes... And this is a culture for cell production not for protein...
08 August 2018 2,051 2 View
Can we freeze the cell lysate and use it later on for RNA isolation? Would the amount/quality get affected if we do so?
08 August 2018 2,335 0 View
I have adherent cells in a 24 well plate I'd like to incubate with a sample for several time intervals before extracting total RNA for RNA-seq. Is it better to stagger the incubation start times...
07 July 2018 5,741 2 View
Good day, my question is fairly easy and I just want to be very careful for my cultured cells. My cells required 90% FBS and 10% DMSO as freezing media. For this, I would need to add the following...
07 July 2018 9,460 7 View
Hello friends, Is it okay to use regular tissue culture/cell culture 96 well plates for measuring absorbance at 450nm? Although the wave length is in visible range I am bit skeptical about that...
07 July 2018 6,623 2 View