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Questions related to Culture Cells
Is it by preparing it with the culture medium or simply adding the desired dosage to the culture plates?
07 July 2018 8,234 0 View
Hi everyone, I am working on cell free supernatants from probiotics, therefore I wish to know how long and at what condition (4 or -20 oC) can I store my CFS prior to use after preparation such...
07 July 2018 4,500 0 View
I am planning to establish primary endothelial cell culture from HUVEC and then to treat them with Dengue virus infected serum for further experiment. I need to confirm my cultured cell as...
24 June 2018 886 5 View
06 June 2018 9,305 6 View
I have a student who has tried on numerous occasions to propagate MB-231s without success. So far we purchased three different batches of these cells from ATCC… We sequentially cultured the cells...
06 June 2018 2,795 2 View
Hi, I have a question for someone with experience in cancer cell metabolism. I wonder how much iron ions are in cultured cells [free or other wise available for haeme synethesis] using 10% FBS. I...
23 May 2018 5,010 4 View
How easy is this to do for someone who has never done this before? We have plenty of experience culturing cells but how easy is it to recognize when it has worked? And does anyone have a simple,...
09 May 2018 3,943 3 View
05 May 2018 3,058 4 View
I am culturing cells in Medium 199 (Sigma, M3769) and the supernatant turns black once the cells are added. There does not appear to be contamination but curious if it could be a chemical reaction.
05 May 2018 2,285 0 View
I would research about colon. So i am going to do primary cell culture. when I searched "mouse colon cell primary culture", but results show me (small) intestinal epithelial cell primary culture...
05 May 2018 6,247 0 View
I'm culturing T cells with mytomycin treated splenocyte as APCs. After 3 days, I want to collect cultured cells for rt-qPCR. I don't know if I need to get rid of APCs so that they wouldn't...
05 May 2018 9,104 1 View
I'm trying to culture adhesion-dependent cell(satellite cell) in suspension. I found a protocol that seeded satellite cell in petri dish for suspension culture, but I wonder if it was surface...
05 May 2018 7,870 9 View
Hi everyone, I want to produce conditioned media from C26 cells for then treat C2C12 myotubes with this conditioned media. I have read some papers using serum-free media for 24h to produce C26...
05 May 2018 7,780 0 View
05 May 2018 1,701 0 View
05 May 2018 4,564 5 View
I am trying to grow a caco-2 cell monolayer on 96-well transwell insert plate (1 micron pore size) with a feeder tray at the bottom. The cell monolayer is grown for 21-23 days to allow tight...
05 May 2018 6,936 5 View
03 May 2018 5,181 1 View
I generally use antibiotics for routine culture of cells but don't use it while doing MTS assay. I am not sure if use of antibiotics should give me aberrant IC50 value of drugs? Any...
27 April 2018 7,009 5 View
I have done previously ROS by DHE stain using flowcytometry. My study design included adherent cell culture: Cells after seeding, treatment, trypsinizing, pellet was incubated with FBS-free medium...
23 April 2018 10,051 9 View
04 April 2018 3,482 6 View
Hi! We are isolating mitochondria from platelets using nitrogen cavitation via a Parr bomb apparatus. Has anyone had experience isolating mitochondria from blood or other culture cells using...
04 April 2018 9,219 1 View
04 April 2018 5,441 10 View
For a metabolic study or to culture these cells
04 April 2018 5,406 1 View
I am culturing cells in a microfluidic device for several days. Before the experiment, I am performing a Nitrogen cavitation procedure in order to expose the intracellular compartments of the...
16 March 2018 6,071 3 View