I'm using Altogen's MDCK-optimized transfection reagent—a polymer-based kit that includes a Transfection Enhancer and Complex Condenser. The official protocol yields ≥ 73% siRNA knockdown in qRT-PCR assays. Be sure to seed MDCK cells 12–24 h before transfection, and while the Enhancer and Condenser can boost efficiency, they may increase cytotoxicity, so titration is key. Overall feedback is that it performs well; just optimize for your constructs and monitor cell health.