0.01 mg/ml fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin.
Hello Pranav SAMIR Kulkarni
You may follow the below protocol.
1. Prepare the mix containing 0.01mg/ml fibronectin, 0.03mg/ml collagen type I and 0.01mg/ml BSA. Ensure that all the components are sterile.
2. Pipette the desired volume of this coating solution (say, 1ml for a T25cm^2 flask) to cover the entire surface of the flask.
3. Place the flask in the incubator at 37 deg C to allow the proteins to bind to the surface.
4. After the incubation period, the excess coating solution may be removed by aspiration. Alternatively, you may allow the flask to dry at room temperature for 45mins to 1 hour.
5. Introduce your cells to the precoated flask and continue culturing your cells as usual.
Important considerations.
a. Ensure the entire flask is covered by the coating solution for consistent cell attachment.
b. Maintain sterility throughout the process to prevent contamination.
Best,
thank you for the answer, can you tell me how to prepare 0.01mg/ml fibronectin, 0.03mg/ml collagen type I and 0.01mg/ml BSA.?
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