In principle, you can generate a standard curve using generic human IgG, but the results should be interpreted with caution. ELISA signal depends not only on the total IgG concentration but also on the specific binding affinity of the antibody in your serum to the coated antigen. A standard curve of unrelated IgG does not fully account for this, so your quantification would be semi-quantitative at best.

If your goal is to measure relative antibody levels between samples (e.g., comparing groups), this approach can still be informative. However, for accurate quantification of antigen-specific antibodies, it is preferable to use a purified antigen-specific antibody (if available) as the standard.

So: yes, you can use generic IgG for a standard curve, but the results will reflect relative binding units, not absolute concentrations of the antigen-specific antibody

But I mean I could coat those wells with antigen for that ab right? I'm not using a kit I will immobilize the antigen myself

Hi,

you mean?

1. coat IgG on the plate -> block-> wash -> secondary antibody-> wash and read

right?

and then have a standard in absolute values

-I think you can use it, but you must enough below the maximum binding capacity of the plate

-you must prove that most antibodies are coated to the surface during the process

-the blocking should not block binding sites for your secondary antibody