Can I use CellTiter Glo 2.0 from Promega with PBS or it's media-only?

16 January 2018 1 4K Report

I just really don't know why my results isn't equal when I use CellTiter-Glo and CellTiter-Glo 2. Can someone explain it to me?

Jordan R. Yaron

Should be okay so long as the cells don't sit in PBS for very long, because you don't want to affect the metabolism of your cells. I used to regularly measure my cells in a home-made HEPES (Mg, Ca, HEPES, Gluc, Pyruv) buffer that contained 0.1% BSA and saw no issues with reliability using CTGlo2.0.

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