What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

08 March 2020 997 3 View

How many base pairs mismatches should i consider to design a specific primer? my templates have 1-2base pairs mismatch only when i aligned them?

i need to design a specific primer for each of my samples. but my samples, when i aligned them, they only have 1-2 bp mismatches, barely 3

17 September 2019 6,650 3 View

Can you recommend a fluorescent ELISA agent?

Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...

07 August 2019 2,604 5 View

What are the Arch Garch conditions?

20 April 2019 3,704 4 View

What is a best way to immobilize RGD peptide with electrospun nanofiber?

I synthesized the nanofiber via electrospinning but need to find a best way to immobilize RGD peptide (Arg-Gly-Asp) onto naofibers.

03 September 2018 8,448 1 View

How to run longitudinal mediation with multiple (5 waves)?

Hi everyone! I'm doing a PhD in Clinical Psychology and I have some treatment data to analyse. The design is a 2 (condition: self-compassion, cognitive restructuring) x 5 (time: baseline,...

01 August 2018 4,502 3 View

Increased thermopower (Seebeck coefficient) with rising temperature? (p-type semiconductor). Is this the case for any p-type sc? What about n-type?

28 March 2018 4,475 3 View

Why do my western blots look so odd?

The three images attached here are for 3 different proteins probed at the same time. The first one is GAPDH. Does anyone have any idea why (1) Despite reasonable Bradford assay results, GAPDH...

14 January 2018 5,544 3 View

Are plasma protein free cysteines truly free?

free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...

14 December 2017 7,799 13 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View